PMID: 2509422Nov 1, 1989Paper

Sigma H-directed transcription of citG in Bacillus subtilis

Journal of Bacteriology
K M TattiC P Moran

Abstract

The RNA polymerase sigma factor sigma H is essential for the onset of endospore formation in Bacillus subtilis. sigma H also is required for several additional stationary-phase-specific responses, including the normal expression of several genes that are required for the development of competence for DNA uptake. It is necessary to identify the genes that are transcribed by sigma H RNA polymerase (E sigma H) in order to understand the role of this sigma factor during the transition from exponential growth to stationary phase. Feavers et al. (Mol. Gen. Genet. 211:465-471, 1988) proposed that citG, the structural gene for fumarase, is transcribed from two promoters, one of which (citGp2 [P2]) may be used by E sigma H. It is likely that the citGp2 promoter is used by E sigma H because we found that this promoter was used accurately in vitro by E sigma H and directed expression of xylE in vivo. This xylE expression was dependent on spo0H, the structural gene for sigma H, and was independent of the citGp1 promoter. Comparison of the nucleotide sequences of several sigma H-dependent promoters showed that these sequences were similar at two regions approximately 10 and 35 base pairs upstream from the start points of transcription. Thes...Continue Reading

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Citations

Oct 1, 1995·FEMS Microbiology Reviews·U SauerP Dürre
Nov 1, 1990·Molecular Microbiology·P Stragier, R Losick
Jun 1, 2010·Journal of Bacteriology·Prahathees EswaramoorthyMasaya Fujita
Dec 16, 1998·Journal of Bacteriology·K DrzewieckiM Hecker
Aug 10, 2002·Journal of Bacteriology·Robert A BrittonAlan D Grossman
Mar 1, 1995·Microbiological Reviews·W G Haldenwang
Jun 1, 1995·Applied and Environmental Microbiology·K MitsushimaS Yagi
Sep 1, 1991·Research in Microbiology·I SmithN Gaur
Sep 1, 1990·Journal of Bacteriology·U BaiI Smith
Jan 1, 1991·Journal of Bacteriology·J WeirI Smith
Nov 7, 1996·Chemical Reviews·Dennis H. Flint, Ronda M. Allen

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