Sigma32-mediated negative regulation of Salmonella pathogenicity island 1 expression.

Journal of Bacteriology
Mari MatsuiTomoko Yamamoto

Abstract

Salmonella pathogenicity island 1 (SPI1) enables infecting salmonellae to invade the intestinal epithelium and induce a proinflammatory response and macrophage cell death. SPI1 expression is controlled by a complex cascade with several transcriptional regulators within the island and global regulators outside it. Previously, we reported that DnaK-depleted salmonellae could neither invade epithelial cells nor secrete SPI1-encoded proteins, suggesting that DnaK is involved in the expression of SPI1. Here, we found that DnaK is involved in SPI1 expression through inhibition of sigma(32) protein, which directs the transcription of a group of genes in response to various global stresses. Overproduction of sigma(32) resulted in decreased levels of the SPI1-specific transcriptional regulators HilD and HilA. Further analysis demonstrated that the sigma(32)-mediated system negatively regulates HilD and HilA at the posttranslational and transcriptional levels, respectively. The executioner of this negative regulation was shown to be a sigma(32)-induced protein ATP-dependent Lon protease, which specifically degrades HilD. Since HilD can activate hilA transcription, is at the top of the hierarchical SPI1 regulatory loop, and has a dominant...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C A LeeS Falkow
May 11, 1990·Science·N A Buchmeier, F Heffron
Mar 1, 1989·Journal of Bacteriology·K TillyC Georgopoulos
Dec 1, 1987·Infection and Immunity·P A Gulig, R Curtiss
Jan 1, 1993·Annual Review of Microbiology·T YuraH Mori
Apr 1, 1995·Infection and Immunity·J Lin, T A Ficht
Jan 1, 1994·Microbiology and Immunology·T YamamotoS Ogata
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K Liberek, C Georgopoulos
Jul 1, 1993·Journal of Bacteriology·I Behlau, S I Miller
Apr 1, 1993·Infection and Immunity·Y Abu KwaikN C Engleberg
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·D M MonackS Falkow
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·D HershA Zychlinsky
Mar 6, 1999·Current Opinion in Microbiology·C Herman, R D'Ari
Sep 16, 1999·The EMBO Journal·R D Hayward, V Koronakis
Dec 1, 1999·Cell Death and Differentiation·A ZeunerR De Maria
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jun 9, 2000·Molecular Microbiology·T S Wallis, E E Galyov
Oct 4, 2000·The Journal of Experimental Medicine·V JesenbergerM Baccarini
Oct 13, 2000·Molecular Microbiology·M A Brennan, B T Cookson
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·C A LeeB A McCormick
Jun 22, 2001·Microbes and Infection·I Hansen-Wester, M Hensel
Jan 5, 2002·Microbes and Infection·C P Lostroh, C A Lee

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Citations

Dec 17, 2009·Genetics·Javier López-Garrido, Josep Casadesús
Jan 5, 2012·Nihon saikingaku zasshi. Japanese journal of bacteriology·Tomoko Yamamoto
Nov 30, 2010·Comparative Immunology, Microbiology and Infectious Diseases·Jin HurJohn Hwa Lee
Nov 5, 2016·Molecular Plant-microbe Interactions : MPMI·Tianhong ZhouXin Deng
Jun 2, 2015·Nihon saikingaku zasshi. Japanese journal of bacteriology·Takeshi ShimizuMasanori Hatakeyama
Apr 5, 2013·Clinical Microbiology Reviews·Anna Fàbrega, Jordi Vila
Sep 30, 2020·Antimicrobial Agents and Chemotherapy·Jitender YadavAyub Qadri
Jun 25, 2021·Frontiers in Molecular Biosciences·Matthias P Mayer

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