Signal amplification of target protein on heparin glycan microarray.

Analytical Biochemistry
Tae-Joon ParkRobert J Linhardt

Abstract

A heparin glycan chip (HepGlyChip) with a 4800-fold enhanced signal-to-noise ratio as compared with the control without heparin was developed for high-throughput analysis of heparin-protein interactions for new drug development and for screening biological samples in diagnostic applications. As a proof of concept, a heparin glycan microarray was prepared on a poly(styrene-co-maleic anhydride) (PS-MA)-coated glass slide. Heparin was covalently immobilized on poly-l-lysine (PLL) layer with multiple binding sites by sulfo-ethylene glycol bis(succinimidylsuccinate) (sulfo-EGS), increasing the signal-to-noise ratio, minimizing nonspecific binding of target proteins, and resulting in a three-dimensional (3D) structure on the HepGlyChip. This on-chip signal amplification platform was successfully demonstrated by probing the heparin microarray with the highly specific heparin-binding protein antithrombin III (AT III).

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Citations

Nov 4, 2011·Applied Microbiology and Biotechnology·Ujjwal BhaskarRobert J Linhardt
Apr 17, 2015·Glycoconjugate Journal·Xuezheng SongRichard D Cummings
Mar 8, 2013·Carbohydrate Polymers·Anaïs A E ChavarocheGerrit Eggink
Jun 27, 2017·Journal of the American Chemical Society·Chengli ZongGeert-Jan Boons
Oct 24, 2019·Chemistry : a European Journal·Cecilia García-OlivaMaría J Hernáiz
Feb 19, 2013·Expert Review of Proteomics·Bernd GesslbauerAndreas J Kungl
Aug 11, 2018·Applied Microbiology and Biotechnology·Svenja Nicolin BoltenThomas Scheper
Jul 29, 2020·Frontiers in Chemistry·Qing ZhangXuezheng Song
Dec 19, 2020·The Journal of Organic Chemistry·Lifeng SunGeert-Jan Boons

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