Signal and Charge Enhancement for Protein Analysis by Liquid Chromatography-Mass Spectrometry with Desorption Electrospray Ionization

International Journal of Mass Spectrometry
Yan LiuHao Chen

Abstract

We recently reported the use of desorption electrospray ionization (DESI) as a novel interface to couple high-performance liquid chromatography (HPLC) with mass spectrometry (MS) (Chem. Commun. 2011, 47, 4171). One of the benefits of such an interface is that post-column derivatization of separated analytes can be integrated with ionization via a "reactive" DESI approach in which a derivatizing reagent is doped into the spray solvent. The reactive DESI interface allows analyte desorption/ionization from the end of the chromatographic column with prompt MS detection; a short time delay of ~20 ms was demonstrated. In this study, we extended this application by "supercharging" proteins following HPLC separation using a DESI spray solvent containing supercharging reagents, m-nitrobenzyl alcohol (m-NBA) or sulfolane. Proteins (insulin, ubiquitin, lysozyme and α-lactalbumin) eluted out of the LC column can be supercharged with the protein charge state distributions (CSDs) significantly increased (to higher charge), which would be advantageous for subsequent top-down MS analysis of proteins. Interestingly, supercharging combined with reactive DESI enhances tolerance towards trifluoroacetic acid (TFA), which is known to be a superior a...Continue Reading

Citations

Aug 19, 2014·The Analyst·Catherine A Cassou, Evan R Williams
Feb 3, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Yi CaiHao Chen
Aug 27, 2015·European Journal of Mass Spectrometry·Yi CaiHao Chen
May 6, 2016·Annual Review of Analytical Chemistry·Qiuling Zheng, Hao Chen
Jan 9, 2019·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Sean P Cleary, James S Prell
Feb 23, 2019·Rapid Communications in Mass Spectrometry : RCM·Durga D KhanalKevin A Schug
Jul 31, 2013·The Analyst·Pengyuan LiuHao Chen
Oct 6, 2016·The Analyst·Shuting XuHuwei Liu
May 12, 2018·International Journal of Mass Spectrometry·Michael NshanianJoseph A Loo
Nov 21, 2013·Journal of the American Society for Mass Spectrometry·Yi CaiHao Chen
Jul 16, 2014·Journal of the American Society for Mass Spectrometry·Yi CaiHao Chen
Aug 20, 2014·Journal of the American Society for Mass Spectrometry·Rachel R Ogorzalek LooJoseph A Loo
Aug 6, 2015·Journal of the American Society for Mass Spectrometry·Mei LuHao Chen
Feb 17, 2019·Journal of the American Society for Mass Spectrometry·Roshan JavanshadAndre R Venter
Aug 27, 2016·Journal of the American Society for Mass Spectrometry·Si ChengHao Chen
Oct 6, 2015·Analytical Chemistry·Dmytro S KulykAbraham K Badu-Tawiah
Aug 1, 2018·Analytical Chemistry·Fred A M G van GeenenMichel W F Nielen

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