Signal-off impedimetric immunosensor for the detection of Escherichia coli O157:H7

Scientific Reports
Jingzhuan WanZhiliang Cheng

Abstract

A signal-off impedimetric immune-biosensor based on gold nanoparticle (AuNP)-mediated electron transfer (ET) across a self-assembled monolayer (SAM) was the developed for highly sensitive detection of Escherichia coli O157:H7 bacteria. The biosensor was fabricated by covalently grafting an anti-Escherichia coli O157:H7 antibody onto SAM-modified gold electrodes. Following bacterial capture, the sensor was further modified by the gold nanoparticles (AuNPs). Due to the strong interaction between AuNPs and Escherichia coli O157:H7, AuNPs attached to the surface of the bacteria and acted as ET pathways across the insulating SAMs on the electrode surface, resulting in a significant reduction of the electron transfer resistance (Ret) between the [Fe(CN)6](3-/4-) redox probe in the solution and the substrate gold surface. Therefore, the attachment AuNPs to captured bacteria significantly enhanced the sensitivity for Escherichia coli O157:H7 bacteria detection.

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Citations

Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Sep 15, 2017·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Prakash Chandra MondalMichael Zharnikov
Jan 12, 2021·Biosensors & Bioelectronics·Madison E StrongJeffrey T La Belle
May 5, 2020·Biosensors & Bioelectronics·Ellen Cesewski, Blake N Johnson
Mar 19, 2021·ACS Infectious Diseases·Carly DeusenberyAnita Shukla
Jan 21, 2017·Analytical Chemistry·Wei WenYuehe Lin
Apr 18, 2020·ACS Applied Materials & Interfaces·Roozbeh Siavash MoakharSara Mahshid

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
biosensors
biosensor
transmission electron microscopy

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