Signal-on photoelectrochemical immunoassay mediated by the etching reaction of oxygen/phosphorus co-doped g-C3N4/AgBr/MnO2 nanohybrids.

Analytica Chimica Acta
Zijian LvDianping Tang

Abstract

We designed a signal-on photoelectrochemical (PEC) immunoassay for the sensitive monitoring of prostate-specific antigen (PSA) based on the etching reaction of hydrogen peroxide (H2O2) toward oxygen/phosphorus co-doped graphitic C3N4/AgBr/MnO2 nanosheets (OP-g-C3N4/AgBr/MnO2). Initially, glucose oxidase (GOX)-labeled detection antibodies were introduced into the capture antibody-coated microplate with a sandwich-type immunoreaction in the presence of PSA. Then, the as-generated H2O2 from the decomposition of glucose by GOX etched the manganese dioxide (MnO2) nanosheets into manganese ions (Mn2+), thereby causing the exposure of the underlying OP-g-C3N4/AgBr. Meanwhile, H2O2 could be also used as an electron scavenger, and restrain the recombination of the electron-hole pairs of OP-g-C3N4/AgBr. Two advantages of H2O2 enhanced the photocurrent synergistically. Under optimum conditions, the PEC immunoassay showed high sensitivity toward target PSA within a dynamic working range of 0.05-50 ng mL-1 with a limit of detection of 17 pg mL-1. In addition, our system possessed high specificity, favorable selectivity, and good stability. Relative to commercialized PSA ELISA kits, the accuracy of our strategy was acceptable. More important...Continue Reading

References

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Dec 10, 2019·Analytical Chemistry·Yong-Quan YangYang-Hsiang Chan
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