PMID: 12633879Mar 14, 2003Paper

Signal peptide of Lassa virus glycoprotein GP-C exhibits an unusual length

FEBS Letters
Robert EichlerWolfgang Garten

Abstract

Lassa virus glycoprotein is synthesized as precursor GP-C into the lumen of the endoplasmic reticulum and cleaved posttranslationally into the N-terminal subunit GP-1 and the C-terminal subunit GP-2 by subtilase SKI-1/S1P. The N-terminal portion of the primary translation product preGP-C contains a signal peptide of unknown length. In order to demonstrate the signal peptide cleavage site, purified viral GP-1 isolated from Lassa virus particles was N-terminally sequenced as TSLYKGV, identical to amino acids 59-65 of GP-C. Mutational analysis of the amino acid residues flanking the putative cleavage site led to non-cleavable preGP-C indicating that no other signal peptide cleavage site exists. Interestingly, GP-C mutants with a non-cleavable signal peptide were not further processed by SKI-1/S1P. This observation suggests that the signal peptide cleavage is necessary for GP-C maturation and hence for Lassa virus replication.

References

Mar 1, 1987·The Journal of Infectious Diseases·J B McCormickE S Smith
Mar 1, 1987·The Journal of Infectious Diseases·J B McCormickT C Tong
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y LiC Y Kang
Jan 10, 1998·The EMBO Journal·B MartoglioB Dobberstein
Oct 28, 1998·Trends in Cell Biology·B Martoglio, B Dobberstein
Mar 17, 2001·Tropical Medicine & International Health : TM & IH·J ter MeulenW Aldis
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·O LenzW Garten

❮ Previous
Next ❯

Citations

Dec 18, 2012·Expert Review of Anti-infective Therapy·Antonella PasquatoStefan Kunz
Aug 15, 2003·The Journal of Biological Chemistry·Marc FroeschkeBernhard Dobberstein
Jan 29, 2010·Journal of Virology·Allison GrosethStephan Becker
Aug 6, 2010·Journal of Virology·Sheli R RadoshitzkySina Bavari
May 14, 2008·The Journal of Biological Chemistry·Andrew M LeeStefan Kunz
Dec 4, 2012·Viruses·Sarah Katharina FehlingThomas Strecker
Feb 26, 2013·Viruses·Svenja WolffAllison Groseth
Jan 10, 2008·Cellular Microbiology·Jillian M Rojek, Stefan Kunz
Jun 19, 2009·Briefings in Bioinformatics·Jan A Hiss, Gisbert Schneider
May 23, 2012·Expert Opinion on Drug Discovery·Sheli R RadoshitzkySina Bavari
Apr 26, 2016·Nature Structural & Molecular Biology·Kathryn M HastieErica Ollmann Saphire
Jan 15, 2010·Journal of Virology·Katrin SchlieWolfgang Garten
Jun 3, 2017·Science·Kathryn M HastieErica Ollmann Saphire
Dec 4, 2012·Viruses·Dominique J BurriAntonella Pasquato
Aug 27, 2016·Virologica Sinica·Wei WangGengfu Xiao
Oct 19, 2004·Critical Reviews in Clinical Laboratory Sciences·Stephan Günther, Oliver Lenz
Jul 25, 2009·Journal of Virology·Sudhakar S AgnihothramJack H Nunberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.