Signal-regulated Pre-mRNA occupancy by the general splicing factor U2AF.

PloS One
Anne Tisserant, Harald König

Abstract

Alternative splicing of transcripts in a signal-dependent manner has emerged as an important concept to ensure appropriate expression of splice variants under different conditions. Binding of the general splicing factor U2AF to splice sites preceding alternatively spliced exons has been suggested to be an important step for splice site recognition. For splicing to proceed, U2AF has to be replaced by other factors. We show here that U2AF interacts with the signal-dependent splice regulator Sam68 and that forced expression of Sam68 results in enhanced binding of the U2AF65 subunit to an alternatively spliced pre-mRNA sequence in vivo. Conversely, the rapid signal-induced and phosphorylation-dependent interference with Sam68 binding to RNA was accompanied by reduced pre-mRNA occupancy of U2AF in vivo. Our data suggest that Sam68 can affect splice site occupancy by U2AF in signal-dependent splicing. We propose that the induced release of U2AF from pre-mRNA provides a regulatory step to control alternative splicing.

References

Jun 17, 1994·Cell·P A Sharp
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·U GosslarB Holzmann
May 10, 2000·Current Opinion in Cell Biology·R Reed
Mar 10, 2001·Nature·L Chang, M Karin
Jun 26, 2001·RNA·B R GraveleyT Maniatis
Aug 3, 2001·The EMBO Journal·S Weg-RemersH König
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Somashe NiranjanakumariMariano A Garcia-Blanco
Jul 12, 2002·Nature·Tom Maniatis, Bosiljka Tasic
Sep 7, 2002·Molecular and Cellular Biology·A AudibertF Dautry
Dec 13, 2002·Nature·Nathalie MatterHarald König
Jan 4, 2003·Nature Reviews. Molecular Cell Biology·Helmut PontaPeter A Herrlich
Feb 26, 2003·Genes & Development·Nuno André Faustino, Thomas A Cooper
Jul 30, 2003·Cancer Metastasis Reviews·Kaladhar B ReddyNatasha Atanaskova
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Chanseok Shin, James L Manley
Sep 2, 2004·Nature Genetics·Heebal KimJurg Ott
Jun 16, 2005·Nature Reviews. Molecular Cell Biology·Arianne J MatlinChristopher W J Smith
Jul 15, 2006·Cell·Benjamin J Blencowe
Aug 31, 2006·Molecular and Cellular Biology·Teresa R PachecoMaria Carmo-Fonseca
Dec 13, 2006·Nucleic Acids Research·Eddo KimGil Ast

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Citations

Oct 1, 2010·Human Molecular Genetics·Enrica BianchiClaudio Sette
Feb 17, 2009·The Journal of Biological Chemistry·Rebeccah J KatzenbergerDavid A Wassarman
Feb 27, 2009·RNA·Mafalda Martins de AraújoJuan Valcárcel
Sep 24, 2009·Nature Reviews. Molecular Cell Biology·Mo Chen, James L Manley
Jul 9, 2010·The Journal of Biological Chemistry·N Helge MeyerMichael Sattler
Oct 29, 2010·The Journal of Biological Chemistry·Lulzim ShkretaBenoit Chabot
Nov 30, 2013·International Journal of Molecular Sciences·Flora Sánchez-Jiménez, Víctor Sánchez-Margalet
Apr 22, 2009·The Journal of Cell Biology·Maria Paola ParonettoClaudio Sette
Jan 14, 2016·Nature Communications·Mikael FeracciCyril Dominguez
Sep 16, 2015·Cellular and Molecular Life Sciences : CMLS·Muhammad Sohail, Jiuyong Xie
Sep 27, 2013·International Journal of Cell Biology·Chiara Naro, Claudio Sette
Aug 15, 2015·BioMed Research International·Paola FrisoneMaria Paola Paronetto
Feb 16, 2019·Nucleic Acids Research·Suryasree SubramaniaMarc-Étienne Huot
May 14, 2011·Endocrine-related Cancer·Pamela BielliClaudio Sette
Sep 22, 2017·Nucleic Acids Research·Naoko KajitaniStefan Schwartz

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Methods Mentioned

BETA
pull-down
co-immunoprecipitations
Co-immunoprecipitation
electrophoretic mobility shift
electrophoretic mobility shift assay
transfections
PCR
immunoprecipitations
immunoprecipitation
CoIP

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