Signal sequence of alkaline phosphatase of Escherichia coli.

Journal of Bacteriology
H InouyeJ Beckwith

Abstract

The amino acid sequence of the signal sequence of phoA was determined by DNA sequencing by using the dideoxy chain termination technique (Sanger et al., Proc. Natl. Acad. Sci. U.S.A. 74:5463-5467, 1977). The template used was single-stranded DNA obtained from M13 on f1 phage derivatives carrying phoA, constructed by in vitro recombination. The results confirm the sequence of the first five amino acids determined by Sarthy et al. (J. Bacteriol. 139:932-939, 1979) and extend the sequence in the same reading frame into the amino terminal region of the mature alkaline phosphatase (Bradshaw et al., Proc. Natl. Acad. Sci. U.S.A., 78:3473-3477, 1981). As was predicted (Inouye and Beckwith, Proc. Natl. Acad. Sci. U.S.A. 74:1440-1444, 1977), the signal sequence was highly hydrophobic. The alteration of DNA sequence was identified for a promoter mutation that results in the expression of phoA independent of the positive control gene phoB and in insensitivity to high phosphate.

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Citations

Mar 1, 1989·Journal of Cellular Biochemistry·J R VasquezC S Craik
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May 1, 2007·Applied Microbiology and Biotechnology·Yanhua FanYan Pei
Dec 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Taehyun ParkRy Young
Apr 20, 2018·Nature·Xianqiang SongEric Gouaux
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