PMID: 2553748Nov 1, 1989Paper

Signal transduction by epidermal growth factor occurs through the subclass of high affinity receptors

The Journal of Cell Biology
L H DefizeS W de Laat

Abstract

Many cell types display two classes of epidermal growth factor receptor (EGFR) as judged from EGF binding studies; i.e., a major class of low affinity EGFR and a minor class of high affinity EGFR. We have studied their respective contribution to the cascade of events elicited by EGF in human A431 carcinoma cells, using anti-EGFR mAb 2E9. This antibody specifically blocks EGF binding to low affinity EGFR, without activating receptors in intact cells, and thus enables us to study the effects of exclusive EGF binding to high affinity EGFR. We show that blocking of low affinity EGFR by mAb 2E9 has almost no effect on the activation of the receptor protein-tyrosine kinase by EGF, suggesting that EGFR kinase activation occurs exclusively through the subclass of high affinity EGFR (5-10%). In addition, we provide evidence that high affinity EGFR exists both in monomeric and dimeric forms, and that cross-phosphorylation of low affinity EGFR by high affinity EGFR may take place in dimers of both receptor types. We demonstrate that the following early cellular response to EGF are also unimpaired in the presence of mAb 2E9: (a) inositol phosphate production, (b) release of Ca2+ from intracellular stores, (c) rise in intracellular pH, (d) ...Continue Reading

References

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Y ShechterP Cuatrecasas
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R N FabricantG J Todaro
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M HoneggerJ Schlessinger
Jan 1, 1986·Annual Review of Physiology·W H Moolenaar
Jan 1, 1987·Annual Review of Biochemistry·G Carpenter
Jan 1, 1987·Journal of Cellular Physiology. Supplement·G N GillP J Bertics
Oct 4, 1984·Nature·J DownwardM D Waterfield
Nov 15, 1984·Archives of Biochemistry and Biophysics·B O FangerJ A Cidlowski
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A B SchreiberJ Schlessinger
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T KawamotoJ Mendelsohn
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B FriedmanM R Rosner

❮ Previous
Next ❯

Citations

Feb 14, 1998·International Journal of Cancer. Journal International Du Cancer·E GenerschR B Lichtner
Sep 25, 1999·Journal of Cellular Physiology·M E HobertC R Carlin
Sep 1, 1992·Journal of Cellular Physiology·S Z HaslamK A Nummy
Apr 1, 1991·Molecular and Cellular Endocrinology·N GentyJ Garnier
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R L WoltjerJ V Staros
Oct 1, 1992·The Journal of Cell Biology·J C den HartighJ Boonstra
Jul 18, 2008·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G MilanoJ-L Formento
Jun 5, 2002·Plastic and Reconstructive Surgery·Roger E De Filippo, Anthony Atala
Jan 8, 2008·Anti-cancer Drugs·Fernando RiveraMarta Francisca López-Brea
Mar 30, 2011·Molecular and Cellular Biology·Christopher J TynanMarisa L Martin-Fernandez
Dec 4, 2012·International Journal of Molecular Sciences·Marisa L Martin-Fernandez, David T Clarke
Jan 1, 1991·Archives of Dermatological Research·M F te PasM Ponec
Dec 22, 2007·Acta Oncologica·Fernando RiveraRaul Marquez
Jan 11, 1991·Growth Factors·R Campos-González, J R Glenney
Oct 16, 2009·Expert Review of Anticancer Therapy·Fernando RiveraLourdes Gutiérrez-Sanz
Apr 22, 2009·Expert Opinion on Biological Therapy·Fernando RiveraCarlos López
Jan 26, 2010·Expert Opinion on Investigational Drugs·Muhammad W Saif
Jun 30, 1997·FEBS Letters·M A Van der HeydenP M Van Bergen en Henegouwen
Oct 21, 2015·Methods : a Companion to Methods in Enzymology·Christopher J TynanMarisa L Martin-Fernandez

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.