Signal transduction events induced by extracellular guanosine 5' triphosphate in excitable cells.

Purinergic Signalling
Tiziana PietrangeloM A Mariggiò

Abstract

A better understanding of the physiological effects of guanosine-based purines should help clarify the complex subject of purinergic signalling. We studied the effect of extracellular guanosine 5' triphosphate (GTP) on the differentiation of two excitable cell lines that both have specific binding sites for GTP: PC12 rat pheochromocytoma cells and C2C12 mouse skeletal muscle cells. PC12 cells can be differentiated into fully functional sympathetic-like neurons with 50-100 ng ml⁻¹ of nerve growth factor, whereas serum starvation causes C2C12 cells to differentiate into myotubes showing functional excitation-contraction coupling, with the expression of myosin heavy chain proteins. Our results show that GTP enhances the differentiation of both of these excitable cell lines. The early events in guanosine-based purine signal transduction appear to involve an increase in intracellular Ca²⁺ levels and membrane hyperpolarization. We further investigated the early activation of extracellular-regulated kinases and phosphoinositide 3-kinase in GTP-stimulated PC12 and C2C12 cells, respectively. We found that GTP promotes the activation of both kinases. Together, our results suggest that, even if there are some differences in the signalling...Continue Reading

References

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Citations

Dec 1, 2011·Purinergic Signalling·Rosa MancinelliStefania Fulle
Jul 2, 2008·Nature Reviews. Drug Discovery·Geoffrey Burnstock
Dec 11, 2013·Tissue Engineering. Part B, Reviews·Serge OstrovidovAli Khademhosseini
Oct 27, 2010·The International Journal of Biochemistry & Cell Biology·Piero Luigi IpataMaria Grazia Tozzi
Dec 30, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S GuarnieriM A Mariggiò
May 20, 2015·Autonomic Neuroscience : Basic & Clinical·George I Gorodeski
Mar 1, 2020·International Journal of Molecular Sciences·Rosa MancinelliStefania Fulle
Jan 1, 1990·International Journal of Immunopathology and Pharmacology·R MancinelliTiziana Pietrangelo
May 15, 2019·Experimental Cell Research·Alessandra BosuttiMarina Sciancalepore

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