Signal transduction in eclosion hormone-induced secretion of ecdysis-triggering hormone

The Journal of Biological Chemistry
T G KinganM E Adams

Abstract

Inka cells of insect epitracheal glands (EGs) secrete preecdysis and ecdysis-triggering hormones (PETH and ETH) at the end of each developmental stage. Both peptides act in the central nervous system to evoke the ecdysis behavioral sequence, a stereotype behavior during which old cuticle is shed. Secretion of ETH is stimulated by a brain neuropeptide, eclosion hormone (EH). EH evokes accumulation of cGMP followed by release of ETH from Inka cells, and exogenous cGMP evokes secretion of ETH. The secretory responses to EH and cGMP are inhibited by the broad-spectrum kinase inhibitor staurosporine, and the response to EH is potentiated by the phosphatase inhibitor calyculin A. Staurosporine did not inhibit EH-evoked accumulation of cGMP. Changes in cytoplasmic Ca2+ in Inka cells during EH signaling were monitored via fluorescence ratioing with fura-2-loaded EGs. Cytoplasmic Ca2+ increases within 30-120 s after addition of EH to EGs, and it remains elevated for at least 10 min, corresponding with the time course of secretion. Secretion is increased in dose-dependent manner by the Ca2+-ATPase inhibitor thapsigargin, a treatment that does not elevate glandular cGMP above basal levels. The secretory response to EH is partially inhibit...Continue Reading

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Citations

May 29, 2003·Journal of Insect Physiology·David B. Morton, P Jeanette Simpson
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jer-Cherng ChangKuang-Hui Lu
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Young-Joon KimDusan Zitnan
Dec 13, 2005·Journal of Insect Science·David B MortonAnke Vermehren
May 18, 2007·General and Comparative Endocrinology·D ZitnanM E Adams
Dec 19, 2012·General and Comparative Endocrinology·Simon George WebsterJasmine Heloise Sharp
Aug 27, 2005·PLoS Biology·Alison R FrandGary Ruvkun
Aug 24, 2002·Journal of Natural Products·Ru Angelie EdradaPeter Proksch

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