Signal transduction mechanism involved in Clostridium perfringens alpha-toxin-induced superoxide anion generation in rabbit neutrophils.

Infection and Immunity
Masataka OdaJ Sakurai

Abstract

Clostridium perfringens alpha-toxin induces the generation of superoxide anion (O2(-)) via production of 1,2-diacylglycerol (DG) in rabbit neutrophils. The mechanism of the generation, however, remains poorly understood. Here we report a novel mechanism for the toxin-induced production of O2(-) in rabbit neutrophils. Treatment of the cells with the toxin resulted in tyrosine phosphorylation of a protein of about 140 kDa. The protein reacted with anti-TrkA (nerve growth factor high-affinity receptor) antibody and bound nerve growth factor. Anti-TrkA antibody inhibited the production of O2(-) and binding of the toxin to the protein. The toxin induced phosphorylation of 3-phosphoinositide-dependent protein kinase 1 (PDK1). K252a, an inhibitor of TrkA receptor, and LY294002, an inhibitor of phosphatidylinositol 3-kinase (PI3K), reduced the toxin-induced production of O2(-) and phosphorylation of PDK1, but not the formation of DG. These inhibitors inhibited the toxin-induced phosphorylation of protein kinase C theta (PKCtheta). U73122, a phospholipase C (PLC) inhibitor, and pertussis toxin inhibited the toxin-induced generation of O2(-) and formation of DG, but not the phosphorylation of PDK1. These observations show that the toxin ...Continue Reading

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Citations

Aug 1, 2012·The Journal of Biological Chemistry·Masataka OdaJun Sakurai
Oct 15, 2009·Future Microbiology·Michel R Popoff, Philippe Bouvet
Sep 29, 2011·Nihon saikingaku zasshi. Japanese journal of bacteriology·Masataka Oda
Dec 4, 2015·Toxins·Masataka OdaMasahiro Nagahama
Oct 29, 2008·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Avraham BeigelmanRachel Levy
Jul 12, 2011·Biochemical and Biophysical Research Communications·Masataka OdaJun Sakurai
May 12, 2017·International Journal of Molecular Sciences·Gaetana MinnoneLuisa Bracci-Laudiero
May 23, 2018·Toxins·Mauricio A NavarroFrancisco A Uzal
May 24, 2013·Microbiology and Molecular Biology Reviews : MMBR·Jihong LiBruce A McClane
Jun 17, 2016·Microbiology and Molecular Biology Reviews : MMBR·Marietta Flores-DíazAntje Flieger
Sep 17, 2008·The Journal of Pharmacology and Experimental Therapeutics·Masataka OdaJun Sakurai
Jul 14, 2017·Scientific Reports·Teruhisa TakagishiMasahiro Nagahama
Jul 28, 2019·Microbiology Spectrum·Masahiro NagahamaJulian I Rood
Oct 29, 2021·FEBS Open Bio·Laura Monturiol-GrossAlberto Alape-Girón

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