Signal transduction pathways involved in mechanical regulation of HB-GAM expression in osteoblastic cells

Biochemical and Biophysical Research Communications
A LiedertA Ignatius

Abstract

Protein kinase C (PKC), protein kinase A (PKA), prostaglandin synthesis, and various mitogen-activated protein kinases (MAPKs) have been reported to be activated in bone cells by mechanical loading. We studied the involvement of these signal transduction pathways in the downregulation of HB-GAM expression in osteoblastic cells after cyclic stretching. Specific antagonists and agonists of these signal transduction pathways were added to cells before loading and to non-loaded control cells. Quantitative RT-PCR was used to evaluate gene expression. The data demonstrated that the extracellular signal-regulated kinase (ERK) 1/2 pathway, PKC, PKA, p38, and c-Jun N-terminal kinase MAPK participated in the mechanical downregulation of HB-GAM expression, whereas prostaglandin synthesis did not seem to be involved.

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Citations

Apr 22, 2014·Interface Focus·Jinju Chen
Sep 21, 2007·Der Internist·F Jakob
Mar 17, 2010·Biochemical and Biophysical Research Communications·Astrid LiedertAnita Ignatius
Jul 7, 2009·Biochemical and Biophysical Research Communications·Astrid LiedertAnita Ignatius
Feb 5, 2008·Biochemical and Biophysical Research Communications·Ludwika KrejaAnita Ignatius
Dec 6, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melanie Haffner-LuntzerMartina Gatzka
Feb 1, 2007·Journal of Cellular Biochemistry·Takahiro KannoTatsuji Nishihara
Aug 2, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Robert Lenartowski, Anna Goc

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