Signal transduction pathways of muscarinic receptor mediated activation in the newborn and adult mouse urinary bladder

BJU International
Mari EkmanAnders Arner

Abstract

To study the role of M(2) and M(3) muscarinic receptor subtypes, sources of activator Ca(2+), and mechanisms involved in increased force oscillations in muscarinic contractions in the bladders of newborn and adult mice, as in the adult bladder muscarinic M(3) receptors are considered to mediate the main part of bladder contraction, and this has not been established in the newborn bladder. Bladder preparations from newborn (0-2 days) and adult (10-12 weeks) mice were mounted for in vitro force registration and activated with carbachol and high-K(+) solution in the presence of M(3) (4-DAMP 30 nM) or M(2) (methoctramine, 100 nM) receptor antagonists. Thapsigargin (1 microm) or ryanodine (10 microm) were used to inhibit sarcoplasmic reticulum Ca(2+) release. L-NAME (300 microm) and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 10 microm) were used to inhibit nitric oxide synthase and guanylyl cyclase, respectively. Gap-junction function was inhibited with by 18-beta-glycyrrhetinic acid (18-beta-GA; 0.1-100 microm). Big-conductance (BK) and small-conductance (SK) K(+) channels were inhibited by apamine and charybdotoxin (0.3 microm), respectively. Concentration-response relations for carbachol in the presence of 4-DAMP and metho...Continue Reading

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Citations

Jun 3, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Thomas J HeppnerMark T Nelson
Dec 30, 2011·American Journal of Physiology. Renal Physiology·John E SpeichPaul H Ratz
Jan 9, 2010·Journal of Applied Physiology·Atheer M AlmasriJohn E Speich
Oct 26, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Sibel BayrakAyşen Erdem
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Feb 1, 2013·The Journal of Pharmacology and Experimental Therapeutics·Koji NobeKazuo Honda
May 25, 2013·American Journal of Physiology. Cell Physiology·Shankar P Parajuli, Georgi V Petkov
Apr 1, 2016·American Journal of Physiology. Renal Physiology·Wenkuan XinGeorgi V Petkov
Jun 20, 2018·Scientific Reports·Caoimhin S GriffinGerard P Sergeant

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