Signal transduction responses following adhesion of verocytotoxin-producing Escherichia coli.

Infection and Immunity
A IsmailiP M Sherman

Abstract

Attaching and effacing adhesion to epithelial cells is a pathognomonic feature of infection by both enteropathogenic Escherichia coli (EPEC) and certain strains of verocytotoxin-producing E. coli (VTEC). EPEC adhesion to tissue culture epithelial cells results in activation of the phosphatidylinositol pathway, with elevated levels of inositol 1,4,5-triphosphate and cytosolic free calcium. In this report, we show that VTEC also activate this signal transduction pathway in infected epithelial cells. Specifically, increased levels of inositol 1,4,5-triphosphate and intracellular free calcium were observed in HEp-2 cells infected with VTEC of serotype O157:H7. VTEC of serotypes O157:H7 and O113:H21 also induced increases in intracellular calcium levels in the human intestinal crypt-like cell line T84, even with minimal or no attaching and effacing activity as monitored by transmission electron microscopy. In contrast to EPEC, VTEC failed to induce tyrosine phosphorylation of epithelial cell proteins in HEp-2 and T84 cells, as determined by indirect immunofluorescence microscopy. These findings suggest that signal transduction responses to VTEC, including elevated levels of inositol triphosphates and intracellular free calcium, are ...Continue Reading

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Citations

Jun 5, 2003·Cellular Microbiology·Jean-Philippe NougayrèdeMichael S Donnenberg
Dec 24, 2014·Infection and Immunity·Steven B LewisStephanie Schüller
May 1, 1997·Journal of Pediatric Gastroenterology and Nutrition·D J PhilpottP M Sherman
Dec 10, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Grace Shen-TuPhilip M Sherman
Nov 26, 2003·Critical Reviews in Microbiology·Jason J LeBlanc
Mar 27, 1999·Journal of Bacteriology·A MatsushiroT Honda
Jul 17, 1999·The American Journal of Physiology·A IsmailiP M Sherman
May 29, 1999·Journal of Bacteriology·F BeltramettiC A Guzmán
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Mar 1, 1997·Trends in Microbiology·M S DonnenbergB B Finlay
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Jul 17, 1998·Clinical Microbiology Reviews·J C Paton, A W Paton
Mar 1, 1996·Microbiological Reviews·C L Sears, J B Kaper
Jun 25, 2015·Microbiology Spectrum·Brian D McWilliams, Alfredo G Torres
Aug 1, 1996·Infection and Immunity·D W AchesonG T Keusch
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper

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