PMID: 8946053Nov 22, 1996Paper

Signaling pathways and survival effects of BDNF and NT-3 on cultured cerebellar granule cells

Brain Research. Developmental Brain Research
T NonomuraH Hatanaka

Abstract

We investigated the signaling pathways exerted by brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in relation to their survival-promoting effects on dissociated cultures of cerebellar granule cells prepared from postnatal 9-day-old rats. Granule neuron survival in culture was supported by BDNF, but not significantly by either nerve growth factor (NGF) or NT-3. BDNF and NT-3 resulted in not only the respective autophosphorylation of the Trk receptors, TrkB or TrkC, but also tyrosine phosphorylation of SHC, a protein involved in controlling p21ras activity, and phosphatidylinositol-3' (PI-3') kinase. NGF does not result in TrkA phosphorylation. In parallel, c-fos was induced within 30 min, in response to BDNF and NT-3. NT-3 induced the phosphorylation of these proteins to a lesser extent than BDNF. BDNF also induced the tyrosine phosphorylation of phospholipase C gamma (PLC gamma), but the NT-3-induced one was not detected. We postulate that no survival promotion by NT-3 is due to lesser level of trkC expression and of the NT-3-induced signaling in the cultured cerebellar granule neurons. Wortmannin, a specific inhibitor of PI-3' inhibited the BDNF effect on neuronal survival. PI-3' kinase-dependent pathways mi...Continue Reading

References

Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K SudaH Thoenen
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M L VetterD R Kaplan
May 1, 1991·Trends in Neurosciences·H Thoenen
Oct 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·V Bocchini, P U Angeletti
May 1, 1994·Trends in Neurosciences·R M LindsayP S DiStefano
Apr 18, 1995·Brain Research. Developmental Brain Research·T KuboH Hatanaka
May 1, 1993·Neuron·D M ValenzuelaG D Yancopoulos
Aug 1, 1993·Trends in Cell Biology·D J Glass, G D Yancopoulos

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Citations

Nov 20, 1998·Journal of Neuroscience Methods·J G Bilsland, S J Harper
Jun 6, 2000·Brain Research. Developmental Brain Research·M B HeatonD W Walker
Jul 18, 1997·Brain Research. Developmental Brain Research·K ShimokeH Hatanaka
Feb 14, 1998·Neurochemistry International·D F CondorelliN Belluardo
Aug 15, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Erin E Rodgers, Anne B Theibert
Feb 24, 2001·Journal of Neurochemistry·C NishijimaY Arakawa
Oct 23, 2010·Cerebral Cortex·Antje GolbsHeiko J Luhmann
Jul 14, 2010·Journal of Neuroscience Methods·Marjo GötteDaniela Gabriel
Aug 20, 2004·Alcoholism, Clinical and Experimental Research·Masaru TatenoToshikazu Saito
Sep 16, 2004·The Journal of Gene Medicine·Jieming ZengShu Wang
Nov 29, 2011·Journal of Neurochemistry·Antje Wagner-Golbs, Heiko J Luhmann
Jul 13, 2004·Neuroscience Letters·Tsuyoshi Oda, Tatsuro Koike
Apr 20, 2006·Neurochemistry International·Ana María Sánchez-PérezVicente Felipo
Oct 13, 2005·Journal of Chemical Neuroanatomy·Ken TakumiMotoharu Hayashi
Oct 17, 2008·International Journal of Cancer. Journal International Du Cancer·Joon Won YoonDavid Walterhouse
Mar 22, 2015·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Yoonyoung ChungYonghyun Jeon
Aug 22, 2009·Expert Opinion on Therapeutic Targets·Michelle K Y SiuAnnie N Y Cheung
Sep 8, 2004·Journal of the Society for Gynecologic Investigation·Jhodie R DuncanSandra M Rees
Jan 1, 2015·Journal of Experimental Neuroscience·Rob U Onyenwoke, Jay E Brenman
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