Signaling pathways involved in human sperm hyperactivated motility stimulated by Zn2

Molecular Reproduction and Development
Deborah Allouche-FitoussiHaim Breitbart

Abstract

To fertilize the egg, sperm cells must reside in the female reproductive tract for several hours during which they undergo chemical and motility changes collectively called capacitation. During capacitation, the sperm develop a unique type of motility known as hyperactivated motility (HAM). The semen contains Zn2+ in millimolar concentrations, whereas in the female reproductive tract, the concentration is around 1 µM. In this study, we characterize the role of Zn2+ in human sperm capacitation focusing on its effect on HAM. Western blot analysis revealed the presence of GPR39-type Zn-receptor localized mainly in the sperm tail. Zn2+ at micromolar concentration stimulates HAM, which is mediated by a cascade involving GPR39-adenylyl cyclase (AC)-cyclic AMP (cAMP)-protein kinase A-tyrosine kinase Src (Src)-epidermal growth factor receptor and phospholipase C. Both the transmembrane AC and the soluble-AC are involved in the stimulation of HAM by Zn2+ . The development of HAM is precisely regulated by cAMP, in which relatively low concentration (5-10 µM) stimulated HAM, whereas at 30 µM no stimulation occurred. A similar response was seen when different concentrations of Zn2+ were added to the cells; low Zn2+ stimulated HAM, whereas ...Continue Reading

References

Sep 1, 1990·Biochimica Et Biophysica Acta·P E ViscontiJ G Tezon
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Nov 1, 1981·Journal of Reproduction and Fertility·S AonumaY Kishi
Aug 1, 1995·The Journal of Cell Biology·L D Walensky, S H Snyder
Jun 2, 1995·The Journal of Biological Chemistry·M L Corcoran, W G Stetler-Stevenson
Dec 1, 1994·Biology of Reproduction·J C AndrewsB D Bavister
Jan 1, 1993·Physiological Reviews·B L Vallee, K H Falchuk
Dec 1, 1995·Biology of Reproduction·C R StaussS S Suarez
Feb 14, 1997·The Journal of Biological Chemistry·D R EmletA J Wong
Aug 29, 1997·The Journal of Biological Chemistry·C W DessauerA G Gilman
Apr 1, 1995·Molecular Medicine Today·G P VinsonJ R Puddefoot
Jul 17, 1998·Molecular Human Reproduction·M B SørensenM Parvinen
Jan 5, 2000·Molecular Reproduction and Development·R A Harrison, N G Miller
May 10, 2000·The Journal of Nutrition·R S MacDonald
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M HershfinkelI Sekler
Jan 24, 2002·The Journal of Biological Chemistry·Claudette KleinAllyn C Howlett
Dec 24, 2002·The Journal of Biological Chemistry·Ignacio A DemarcoPablo E Visconti
Sep 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bijay S Jaiswal, Marco Conti
Nov 25, 2003·Nature Cell Biology·Dan WangDavid L Garbers
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anne E CarlsonDonner F Babcock
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Timothy A QuillDavid L Garbers
Feb 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Samra Mededovic, Lynn R Fraser
Jan 13, 2006·The Journal of Biological Chemistry·María T BranhamClaudia N Tomes
Jul 18, 2006·Developmental Biology·Fang XieMarco Conti
Sep 9, 2006·Endocrinology·Birgitte HolstThue W Schwartz
Dec 22, 2006·Biology of Reproduction·Becky Marquez, Susan S Suarez
May 15, 2007·Biology of Reproduction·Nir EtkovitzHaim Breitbart
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dan WangDavid L Garbers
Sep 22, 2007·Journal of Receptor and Signal Transduction Research·Shin-ichiro YasudaAkihito Kamizono
Feb 13, 2009·Reproduction, Fertility, and Development·Katharine HoSusan S Suarez
Mar 10, 2009·Free Radical Biology & Medicine·Eve de LamirandeMichèle Villemure
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sonoko YamaguchiTakeshi Miura

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Oct 25, 2020·International Journal of Molecular Sciences·Deborah Allouche-Fitoussi, Haim Breitbart

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