Signalling Alterations in Bones of Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Gene Deficient Mice

International Journal of Molecular Sciences
Gergő JózsaT Juhasz

Abstract

: Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with diverse developmental roles, including differentiation of skeletal elements. It is a positive regulatory factor of chondrogenesis and osteogenic differentiation in vitro, but little is known about its in vivo role in bone formation. In our experiments, diaphyses of long bones from hind limbs of PACAP gene-deficient mice showed changes in thickness and increased staining intensity. Our main goal was to perform a detailed morphological and molecular biological analysis of femurs from PACAP knockout (KO) and wild type (WT) mice. Transverse diameter and anterior cortical bone thickness of KO femurs showed significant alterations with disturbed Ca2+ accumulation and collagen type I expression. Higher expression and activity of alkaline phosphatase were also observed, accompanied by increased fragility PACAP KO femurs. Increased expression of the elements of bone morphogenic protein (BMP) and hedgehog signalling was also observed, and are possibly responsible for the compensation mechanism accounting for the slight morphological changes. In summary, our results show that lack of PACAP influences molecular and biomechanical properties of bone matrix, a...Continue Reading

Citations

Dec 7, 2019·Journal of Molecular Neuroscience : MN·Gergő JózsaTamás Juhász
Aug 9, 2020·Frontiers in Endocrinology·Denes TothDora Reglodi
Aug 14, 2020·International Journal of Molecular Sciences·Vince SzegeczkiTamás Juhász
Nov 7, 2020·International Journal of Molecular Sciences·Grazia MaugeriVelia D'Agata
Apr 26, 2021·Journal of Molecular Neuroscience : MN·Timea KvarikTamas Atlasz
Nov 30, 2021·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Samyak Pandey, Jayesh Mudgal

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear translocation
PCR
X-ray
dissection
Assay
electrophoresis

Software Mentioned

NRecon
cellSense Entry
Runx2
Adobe Photoshop
CTAn
ImageJ

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.