Signalling effect of NIR pulsed lasers on axonal growth

Journal of Neuroscience Methods
Manoj MathewPablo Loza-Alvarez

Abstract

In this work we show that a pulsed laser light placed at a distance is able to modulate the growth of axons of primary neuronal cell cultures. In our experiments continuous wave (CW), chopped CW and modelocked fs (FS) laser light was focused through a microscope objective to a point placed at a distance of about 15 microm from the growth cone. We found that CW light does not produce any significant influence on the axon growth. In contrast, when using pulsed light (chopped CW light or FS pulses), the beam was able to modify the trajectory of the axons, attracting approximately 45% of the observed cases to the beam spot. Such effect could possibly indicate the capacity of neurons to interpret the pulsating NIR light as the source of other nearby cells, resulting in extension of processes in the direction of the source.

References

Jun 30, 1992·Journal of Photochemistry and Photobiology. B, Biology·H J Niggli
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G Albrecht-Buehler
Aug 1, 1992·The Journal of Cell Biology·P LamoureuxS R Heidemann
Aug 1, 1991·The Journal of Cell Biology·G Albrecht-Buehler
Aug 5, 1991·Neuroscience Letters·P FromherzT Vetter
Nov 1, 1988·Neuron·T Mitchison, M Kirschner
Jan 1, 1985·Journal of Neuroscience Research·N B PatelM Poo
Jan 1, 1994·Cell Motility and the Cytoskeleton·G Albrecht-Buehler
Jan 1, 1995·Cell Motility and the Cytoskeleton·G Albrecht-Buehler
Jan 1, 1996·Ultrasound in Medicine & Biology·A G Doukas, T J Flotte
Nov 15, 1996·Science·M Tessier-Lavigne, C S Goodman
Apr 16, 1998·Current Biology : CB·G Gallo, P C Letourneau
Feb 19, 2000·Current Opinion in Cell Biology·G G Borisy, T M Svitkina
Oct 12, 2000·Biophysical Journal·T KodamaA G Doukas
May 31, 2001·Science·D PantaloniM F Carlier
Nov 14, 2001·Journal of Photochemistry and Photobiology. B, Biology·H J NiggliL A Applegate
Nov 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·A EhrlicherJ Kas
Dec 10, 2002·Science·Barry J Dickson
Apr 5, 2003·Annual Review of Neuroscience·Andrea B HuberJean-François Cloutier
Apr 22, 2003·Genes & Development·Jeffrey L Goldberg
May 25, 2004·Stroke; a Journal of Cerebral Circulation·Paul A LapchakJustin A Zivin
May 27, 2004·Microbiological Research·Maxim V Trushin
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bryan KaehrJason B Shear
Nov 24, 2004·The Journal of Biological Chemistry·Margaret T T Wong-RileyHarry T Whelan
Mar 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Guenter Albrecht-Buehler
Jul 12, 2005·Experimental Eye Research·Tida Kumbalasiri, Ignacio Provencio
Sep 1, 2005·Annals of Biomedical Engineering·Trent M FischerDavid J Odde
Dec 21, 2005·Journal of Cellular Biochemistry·Andreas W HenkelJens Wiltfang
May 19, 2006·Photomedicine and Laser Surgery·Kristina D DesmetHarry T Whelan
Apr 13, 2007·Bioelectrochemistry·Ashkan FarhadiAli Keshavarzian
Jan 12, 2008·Optics Letters·E N GlezerE Mazur
Apr 5, 2008·Journal of Microscopy·K N Anisha ThayilP Loza-Alvarez
Jul 9, 2008·Optics Express·D J CarnegieK Dholakia
Nov 13, 2008·Cytotechnology·Akon HiguchiYuki Matsuoka
Dec 17, 2008·Cytotechnology·Manoj MathewPablo Loza-Alvarez
Feb 24, 2009·Stroke; a Journal of Cerebral Circulation·Justin A ZivinUNKNOWN NeuroThera Effectiveness and Safety Trial-2 Investigators
Oct 16, 2006·Optics Express·D J StevensonF Gunn-Moore

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Citations

Oct 7, 2011·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Moacyr A Rosa, Sarah H Lisanby
Aug 13, 2011·Journal of the Royal Society, Interface·Tao WuMichael W Berns
Apr 11, 2014·PloS One·Bryan J BlackSamarendra K Mohanty
Nov 16, 2012·Medical Devices : Evidence and Research·Khalid Saad Al-Harbi, Naseem Akhtar Qureshi
Mar 6, 2012·Journal of Neuroscience Methods·Christian L Ebbesen, Henrik Bruus
Mar 5, 2013·Biotechnology and Bioengineering·Chiara PavioloSally L McArthur
Nov 17, 2015·Scientific Reports·Kotaro OyamaShin'ichi Ishiwata
Jul 3, 2013·Optics Letters·B BlackS K Mohanty
Jun 26, 2013·Journal of Biophotonics·Chiara PavioloPaul R Stoddart
Jul 25, 2018·Scientific Reports·Trung Quang PhamAkihito Sano
Dec 7, 2018·Scientific Reports·Remy AvilaPablo Loza-Alvarez
Jan 13, 2018·Applied Optics·José Ángel Picazo-BuenoVicente Micó
Jul 5, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Sahitya Chetan PandanaboinaMalathi Srivatsan
Mar 23, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Yi-Chia ShanJih-Huah Wu

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