PMID: 11606185Oct 19, 2001Paper

Signalling events underlying platelet aggregation induced by the glycoprotein VI agonist convulxin

European Journal of Biochemistry
Ben T AtkinsonS P Watson

Abstract

We have investigated the role of secretion and intracellular signalling events in aggregation induced by the glycoprotein (GP)VI-selective snake venom toxin convulxin and by collagen. We demonstrate that aggregation induced by threshold concentrations of convulxin undergoes synergy with ADP acting via the P2Y12 receptor whereas there is no synergy via the P2Y1 receptor or with thromboxanes. On the other hand, apyrase, the P2Y12 receptor antagonist, AR-C67085, and indomethacin only marginally inhibit aggregation induced by convulxin. In comparison, these inhibitors severely attenuate the response to collagen. In order to investigate whether the weak inhibitory action against convulxin is due to release of agonists other than ADP from dense granules, experiments were performed on murine platelets deficient in this organelle (pearl mice platelets). A slightly greater reduction in aggregation induced by convulxin was observed in pearl platelets than in the presence of inhibitors of ADP, but a maximal response was still attained. Importantly, inhibition of protein kinase C further reduced the response to convulxin in pearl platelets demonstrating a direct role for the kinase in aggregation. Chelation of intracellular Ca2+ with 1,2-b...Continue Reading

References

Jul 26, 1996·The Journal of Biological Chemistry·J GibbinsS P Watson
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Citations

Apr 13, 2011·Purinergic Signalling·Martyn P Mahaut-SmithRichard J Evans
Jan 22, 2004·Critical Reviews in Oncology/hematology·Indra Ramasamy
Apr 15, 2011·The Journal of Biological Chemistry·Amanda J UnsworthCatherine J Pears
Aug 13, 2003·Current Opinion in Hematology·Pamela B Conley, Suzanne M Delaney
Sep 29, 2011·FEBS Letters·Sagit CohenNoah Isakov
May 15, 2012·Cellular Signalling·Margitta ElversKnut Fälker
May 3, 2008·Free Radical Biology & Medicine·Nadia Bakdash, Mark S Williams
Dec 31, 2005·American Journal of Human Genetics·Neil V MorganEamonn R Maher
May 28, 2004·The Journal of Biological Chemistry·Jerzy-Roch NoferBeate E Kehrel
Aug 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pascal MauriceFrançoise Fauvel-Lafeve
Dec 16, 2004·The Journal of Biological Chemistry·Silvia RiondinoFabio M Pulcinelli
Aug 23, 2011·Molecular and Cellular Biochemistry·Milan PopovićEsma R Isenović
Oct 24, 2002·Blood·Margaret J StaffordCatherine J Pears
Mar 22, 2003·Blood·Bernhard Nieswandt, Steve P Watson
Jun 19, 2004·Platelets·Martyn P Mahaut-SmithRichard J Evans
Apr 28, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Alessandro AliottaLorenzo Alberio
Aug 14, 2012·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Hyun-Jeong ChoHwa-Jin Park
Jan 3, 2021·American Journal of Clinical Pathology·Joseph H ChoJonathan L Miller
Aug 4, 2021·Blood Advances·Nathan EatonHervé Falet
Jan 25, 2019·Toxicon : Official Journal of the International Society on Toxinology·Jaqueline Gomes RosaMarcelo Larami Santoro

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