Signalling through either the p38 or ERK mitogen-activated protein (MAP) kinase pathway is obligatory for phorbol ester and T cell receptor complex (TCR-CD3)-stimulated phosphorylation of initiation factor (eIF) 4E in Jurkat T cells

FEBS Letters
S J Morley

Abstract

Initiation factor (elF) 4E plays a key role in the regulation of translation. Its activity is modulated both by phosphorylation and by its association with an inhibitory protein, 4E-BP1, which precludes its interaction with eIF4G. Although increased eIF4E phosphorylation has been correlated with the activation of protein synthesis in T cells, the kinase(s) and/or phosphatase(s) involved have not been characterised. There is evidence for phosphorylation of eIF4E mediated by both protein kinase C-dependent and -independent signalling pathways. In these studies, I show that activation of protein kinase C with phorbol ester, stimulation via the T cell receptor complex with the monoclonal antibody OKT3 and cellular stresses increase the phosphorylation of eIF4E in Jurkat T cells. In contrast to published data, inhibition of either the ERK MAP kinase or p38 MAP kinase signalling pathways does not affect the PMA- or OKT3-stimulated increase in eIF4E phosphorylation. However, simultaneous inhibition of both of these pathways with selective inhibitors is required to completely abrogate the enhanced phosphorylation of eIF4E. These data show that in Jurkat cells, protein kinase C modulates the phosphorylation status of eIF4E indirectly vi...Continue Reading

References

Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Jun 16, 1995·The Journal of Biological Chemistry·B JoshiR E Rhoads
Jan 28, 1994·Cell·A Weiss, D R Littman
Mar 15, 1996·European Journal of Biochemistry·V M Pain
Oct 4, 1996·The Journal of Biological Chemistry·J M Kyriakis, J Avruch
Jun 6, 1997·The Journal of Biological Chemistry·J B CrawleyB M Foxwell

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Citations

Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M GaleM G Katze
Jun 29, 2000·Annual Review of Biochemistry·A C GingrasN Sonenberg
Mar 11, 2009·Journal of Biomedical Science·Xuejuan Gao, Da Xing
Jun 19, 2004·International Reviews of Immunology·Laura Beretta
Apr 23, 2016·Viruses·Elizabeth Royall, Nicolas Locker
Aug 22, 2009·Molecular Reproduction and Development·Corinna SiemerWolfgang Tomek
Aug 2, 2003·American Journal of Physiology. Cell Physiology·Petra RocicPamela A Lucchesi
Jul 16, 2003·The Journal of Biological Chemistry·Petra RocicKathy K Griendling
May 2, 2000·The Journal of Biological Chemistry·P Gómez del ArcoJ M Redondo
Mar 29, 2012·Journal of Cell Science·Yoann RannouClaude Prigent
Jul 6, 2000·Immunopharmacology·J C LeeJ L Adams

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