Signalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress

Scientific Reports
Raúl GarcíaJavier Arroyo

Abstract

Post-transcriptional control of mRNA is a key event in the regulation of gene expression. From yeast to human cells, P-bodies are cytoplasmic RNA-protein aggregates that play an essential role in this process, particularly under stress conditions. In this work, we show that in the model yeast Saccharomyces cerevisiae cell wall stress induces the formation of these structures. This effect is dependent on multiple elements in the Cell Wall Integrity (CWI) MAPK signalling pathway, a signal transduction cascade responsible for the maintenance of cell integrity under adverse environmental conditions. Remarkably, P-body assembly requires the catalytic activity of the MAPK of the pathway, Slt2/Mpk1. In accordance with the control exerted by this signalling pathway, the timing of P-body formation is similar to that of the activation of the CWI pathway. Noticeably, mRNAs whose expression is regulated by this pathway localize in P-bodies after the cell is exposed to stress following a temporal pattern coincident with CWI pathway activation. Moreover, when these mRNAs are overexpressed in a mutant background unable to form visible P-bodies, the cells show hypersensitivity to agents that interfere with cell wall integrity, supporting that ...Continue Reading

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Citations

Jul 1, 2019·Applied Microbiology and Biotechnology·Xueliang QiuGuocheng Du
Feb 6, 2021·Critical Reviews in Biotechnology·Hui LiuLiming Liu
Dec 12, 2021·Critical Reviews in Microbiology·Hajar YaakoubNicolas Papon

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Methods Mentioned

BETA
environmental stress
nucleotide
GTPase
fluorescence microscopy
environmental stresses
Confocal microscopy
PCR
ELISA

Software Mentioned

Fiji
ImageJ
MetaMorph

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