Significance of AHR nuclear translocation sequence in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cPLA2 α activation and hydronephrosis

Archives of Toxicology
Nozomi FujisawaChiharu Tohyama

Abstract

The aryl hydrocarbon receptor (AHR) plays a major role in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced toxicity phenotypes. TCDD bound to AHR elicits both genomic action in which target genes are transcriptionally upregulated and nongenomic action in which cytosolic phospholipase A2α (cPLA2α) is rapidly activated. However, how either of these actions, separately or in combination, induces toxicity phenotypes is largely unknown. In this study, we used AHRnls/nls mice as a model in which AHR was mutated to lack nuclear translocation sequence (NLS), and AHRd/- mice as the corresponding control. Using this model, we studied TCDD-induced alterations in cPLA2α activation and related factors because of the pivotal roles of cPLA2α both in AHR's nongenomic action and in regulation of causative genes of TCDD-induced hydronephrosis. Dams were orally administered TCDD at a dose of 300 µg/kg body weight on postnatal day 1, and pups subsequently exposed to TCDD via milk were examined for gene expression on PND 7 and for histological changes on PND 14. The activation of the AHR genomic action and hydronephrosis onset were observed in the control group but not in the AHRnls/nls group. An ex vivo experiment using peritoneal macrophages ex...Continue Reading

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K M BurbachC A Bradfield
Sep 1, 1973·Environmental Health Perspectives·J A MooreJ G Vos
Feb 16, 1996·The Journal of Biological Chemistry·B N Fukunaga, O Hankinson
Feb 28, 1998·The Journal of Biological Chemistry·T IkutaK Kawajiri
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y ShimizuT Ishikawa
Jun 16, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·P L BryantB D Abbott
Feb 8, 2003·Biochimica Et Biophysica Acta·Junsei Mimura, Yoshiaki Fujii-Kuriyama
Jul 11, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Martin Van den BergRichard E Peterson
Sep 11, 2007·Environment International·Prashant S KulkarniCarlos A M Afonso
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jul 29, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Maureen K BungerChristopher A Bradfield
Oct 29, 2008·Biochemical Pharmacology·Christoph F A Vogel, Fumio Matsumura
Oct 12, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Manabu NukayaChristopher A Bradfield
Dec 15, 2010·Biochimica Et Biophysica Acta·Bin DongChristoph Franz Adam Vogel
Jan 12, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rachel TanosGary H Perdew
Mar 21, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Wataru YoshiokaChiharu Tohyama
Jun 15, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Rachel TanosGary H Perdew
Dec 13, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer L Head WheelerB Paige Lawrence
Jan 22, 2013·Cellular Signalling·Javier Rey-BarrosoPedro M Fernandez-Salguero
Sep 4, 2013·Immunology and Cell Biology·Christoph F A VogelIsaac N Pessah
Jul 13, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Keiko Aida-YasuokaChiharu Tohyama
Jul 22, 2016·American Journal of Physiology. Renal Physiology·Wataru YoshiokaChiharu Tohyama
Apr 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Seung-Hyeon SeokYongna Xing
Nov 2, 2017·Journal of Biochemistry·Ikuru KudoHideaki Itoh

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Methods Mentioned

BETA
nuclear translocation
protein assay

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