Significance of human cytomegalovirus DNA detection in immunocompromised heart transplant patients

Transplantation
C WolffK Kleesier

Abstract

Peripheral blood samples (n= 1240), obtained at variable intervals from 483 heart transplantation (HTx patients under immunosuppressive therapy, and blood samples (n=1013) obtained upon blood donation from 1013 healthy anti-human cytomegalovirus (HCMV) positive blood donors, were tested for HCMV DNA by nested polymerase chain reaction (PCR). The detection limit of the nested PCR was determined to be less than 10 copies of the plasmid pRR 47, containing the HCMV immediate early gene. HCMV DNA was detected in 79 of 483 HTx patient (17%). To the contrary' HCMV DNA was only detected in 1 of 1013 anti-HCMV positive, healthy blood donors (0.1%). This PCR positive donor had recently contracted a primary HCMV infection. The rate of HCMV PCR positive immunosuppressed HTx patients in our study was lower than the rate of HCMV PCR positive healthy blood donors in previous reports in the literature. Blood samples (269 from 117 HTx patients) were assayed for HCMV DNA in peripheral blood leukocytes, HCMV DNA in plasma, and HCMV tegument protein 65 kDa (pp 65 antigen). Three laboratory diagnostic patterns were observed and related to clinical findings: (1) HCMV DNA only in leukocytes was observed in 26 patients, 7 of whom had HCMV disease, 5 o...Continue Reading

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Citations

Feb 19, 2000·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·R HorváthJ Benedík
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·S KusneJ Fung
May 25, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·P Martín-DávilaS Moreno
Feb 22, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Lisbeth BarkholtAnneka Ehrnst
Jul 10, 1999·Biulleten' eksperimental'noĭ biologii i meditsiny·M S DolgikhB Pustovoĭt

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