Significance of molecular identification and antifungal susceptibility of clinically significant yeasts and moulds in a global antifungal surveillance programme.

Mycopathologia
Michael A PfallerMariana Castanheira

Abstract

The increasing diversity of opportunistic fungi causing serious invasive fungal infections (IFI) has been documented. Accurate identification (ID) is important in guiding therapy, determining prognosis for IFIs and in epidemiological surveys. We assessed the utility of PCR-based methods for the ID of yeasts and moulds that either were uncommon, failed conventional ID, or represented unusual biochemical or phenotypic profiles of common species. Among 1,790 viable fungal clinical isolates received during the SENTRY Program in 2010, 322 strains from 40 study sites had ID confirmed by molecular methods. Isolates were previously identified in participant institutions. Yeasts that were not confirmed by morphology on CHROMagar, growth at 45 °C (Candida albicans/dubliniensis), or assimilation of trehalose (C. glabrata) as well as non-Candida yeasts and all moulds were amplified and sequenced using primers amplifying one or more of the following genes: ITS, 28S, β-tubulin (Aspergillus spp.), TEF (Fusarium spp.), IGS (Trichosporon spp.). The isolates selected for molecular ID included 149 isolates of Candida species, 77 of Aspergillus species, 73 non-Candida yeasts, and 23 other moulds (a total of 41 different species). Overall, the ID d...Continue Reading

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Citations

Dec 14, 2018·Mycoses·Leonardo PotenzaLivio Pagano
Feb 19, 2019·Mycoses·Shabir A Lone, Aijaz Ahmad
Dec 19, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shawn R LockhartAnastasia P Litvintseva
Dec 25, 2013·Antimicrobial Agents and Chemotherapy·A F SchmalreckC Lass-Flörl
May 23, 2014·Journal of Clinical Microbiology·Bettina SchulthessMichael Hombach
Sep 20, 2015·Medical Mycology·Michael A Pfaller, Mariana Castanheira
Aug 20, 2014·The Journal of Antimicrobial Chemotherapy·Lívia S RamosAndré L S Santos
Oct 23, 2012·Mycopathologia·Luz Ángela CastroErnesto Martínez
Jan 24, 2016·The Journal of Antimicrobial Chemotherapy·Anna Kołaczkowska, Marcin Kołaczkowski
Mar 22, 2019·Open Forum Infectious Diseases·Michael A PfallerRonald N Jones
Aug 24, 2013·Journal of Clinical Microbiology·Brunella PosteraroMaurizio Sanguinetti
Aug 1, 2018·Antimicrobial Agents and Chemotherapy·Michael A PfallerMariana Castanheira
Aug 6, 2021·Diagnostic Microbiology and Infectious Disease·Michael A PfallerMariana Castanheira

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