Significance of palmitoylation of CD81 on its association with tetraspanin-enriched microdomains and mediating hepatitis C virus cell entry

Virology
Yong-Zhe ZhuZhong-Tian Qi

Abstract

CD81, a co-receptor for hepatitis C virus (HCV), is a member of the tetraspanin superfamily and is heavily palmitoylated in the juxtamembrane cysteine residues. Palmitoylation plays an important role in protein-protein interactions and association with cholesterol-rich domains of membranes. In this study, Huh7 cells expressing wild-type or palmitoylation-defective CD81 were generated to analyze whether palmitoylation of CD81 is involved in HCV cell entry. Our data showed that de-palmitoylation of CD81 dramatically reduced its association with tetraspanin CD151, but did not influence CD81 partition in detergent-resistant membranes. Moreover, de-palmitoylated CD81 decreased the host cell susceptibility to HCV. Notably, CD151-specific antibodies and siRNA inhibited HCV cell entry, and detachment of CD81 with CD151 decreased the lateral movement of virus particle/CD81 complex to areas of cell-cell contact. These results suggest that palmitoylation of CD81 should facilitate HCV entry, at least in part, by regulating the association of CD81 with tetraspanin-enriched microdomains.

References

Mar 29, 2000·The Journal of Biological Chemistry·R L YauchM E Hemler
Aug 16, 2001·The Journal of Biological Chemistry·C S StippM E Hemler
Oct 2, 2001·Cellular and Molecular Life Sciences : CMLS·C Boucheix, E Rubinstein
Jan 5, 2002·The Journal of Cell Biology·M E Hemler
Jan 17, 2002·The New England Journal of Medicine·D J Wyler
Apr 18, 2002·FEBS Letters·Stéphanie CharrinEric Rubinstein
Mar 5, 2003·The Journal of Experimental Medicine·Birke BartoschFrançois-Loïc Cosset
Apr 16, 2003·Biochemistry·Maurine E Linder, Robert J Deschenes
Apr 24, 2003·The Biochemical Journal·Stéphanie CharrinEric Rubinstein
Jul 19, 2005·Physiology·Shoshana Levy, Tsipi Shoham
Apr 28, 2006·Journal of Virology·Claire Bertaux, Tatjana Dragic
Sep 1, 2006·Journal of Virology·Mike FlintJane A McKeating
Sep 8, 2006·Journal of Virology·Jin ZhongFrancis V Chisari
Jul 24, 2007·Molecular & Cellular Proteomics : MCP·Oleg V KovalenkoMartin E Hemler
Oct 19, 2007·Journal of Virology·Sonia MolinaChantal Fournier-Wirth
Nov 16, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mirjam B ZeiselThomas F Baumert
Feb 19, 2008·Biochemical and Biophysical Research Communications·Yanning LuJames P Tam
Apr 3, 2008·PloS One·Vera Rocha-PeruginiLaurence Cocquerel
Jun 4, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Paloma Sanz-CamenoRicardo Moreno-Otero
Jul 16, 2008·Clinics in Liver Disease·Zania StamatakiJane A McKeating
Sep 2, 2008·Nature Reviews. Drug Discovery·Martin E Hemler
Nov 26, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Philip MeulemanGeert Leroux-Roels
Feb 3, 2009·Virology·Gemma C CarterWilliam James
Apr 10, 2009·Journal of Virology·Christopher J MeeJane A McKeating
Sep 19, 2009·The Journal of Biological Chemistry·Stéphanie CharrinOlivier Silvie
Jan 14, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sophie E KriegerThomas F Baumert
Apr 9, 2010·The Journal of Biological Chemistry·Helen J HarrisJane A McKeating
Dec 24, 2010·Journal of Virology·Yimin TongZhong-Tian Qi
Feb 24, 2011·The Journal of Biological Chemistry·Claire MontpellierLaurence Cocquerel

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Citations

Apr 8, 2014·World Journal of Gastroenterology : WJG·Yong-Zhe ZhuZhong-Tian Qi
May 8, 2014·Viruses·Selma DahmanePierre-Emmanuel Milhiet
Apr 5, 2013·The FEBS Journal·Sanja BlaskovicF Gisou van der Goot
Jan 30, 2013·Biochemical Society Transactions·Mathieu BlancF Gisou van der Goot
Feb 18, 2016·The Journal of General Virology·Xi-Jing QianYong-Zhe Zhu
Jan 13, 2017·Molecular & Cellular Proteomics : MCP·Gisa GeroldThomas Pietschmann
Apr 15, 2017·Biochemical Society Transactions·Laura A FastLuise Florin
Feb 8, 2014·Nature Reviews. Cancer·Martin E Hemler
Sep 22, 2019·Molecular Biotechnology·Gayathri Purushothaman, Vijay Thiruvenkatam
Oct 21, 2018·Scientific Reports·Ngan N T NguyenSoon B Hwang
Jun 12, 2018·Frontiers in Immunology·Luise Florin, Thorsten Lang
Mar 18, 2020·The FEBS Journal·Viviana NevianiPiet Gros
May 11, 2021·Frontiers in Immunology·Waqas AhmedHameeda Sultana
Jun 9, 2021·Cell Research·Yuwei HuangHongxia Wang
May 22, 2020·The Journal of Physical Chemistry. B·Ivan Castello-SerranoIlya Levental

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