Abstract
Thromboembolic events (TEE) in patients receiving infusions of intravenous immunoglobulin (IVIG) products have recently been associated with contaminating factor XIa. We studied whether platelet and monocyte activation could also be involved. Twenty IVIG samples from five manufacturers were tested for the induction of visible whole blood clot formation. A selection of TEE-associated and not associated lots was further analyzed for effects on thromboelastometry, platelet activation and adhesion, as well as monocyte tissue factor surface expression. Pure factor XIa was included for comparison. Western blotting was applied to analyze anti-CD154-reactive proteins in IVIG. In whole blood, IVIG enhanced macroscopic clotting additively with factor XIa. In monocytes, all IVIG products induced the FcγRII-dependent tissue factor expression to a similar extent, which was not affected by addition of factor XIa. Testing platelet aggregation, IVIG strengthened the ADP and TRAP-6-elicited response. Furthermore, IVIG increased platelet-monocyte adhesion and annexin V binding to platelet microvesicles, and promoted platelet adhesion to IVIG-coated surfaces. The strongest effects were observed with TEE-associated lots. CD154-related proteins wer...Continue Reading
References
Jul 26, 1986·Lancet·R K WoodruffI L Smith
Feb 1, 1972·British Journal of Haematology·J F MustardM A Packham
Sep 10, 1994·Lancet·P H Bolton-MaggsG S Lucas
Jul 5, 1996·The Journal of Biological Chemistry·B HaimovichR Greco
Aug 11, 2000·American Journal of Hematology·A S WolbergM Hoffman
Sep 26, 2001·Circulation·A D MichelsonM I Furman
Sep 27, 2005·Transfusion·Robert V DiemelRob C Aalberse
Dec 23, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Masashi NambaJunichi Yoshikawa
Dec 8, 2007·Thrombosis and Haemostasis·Benedetta IzziGiovanni de Gaetano
Jun 21, 2008·The British Journal of Dermatology·A PollreiszH J Ankersmit
Aug 8, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Tara C White-AdamsOwen J T McCarty
Nov 6, 2009·Thrombosis and Haemostasis·Eduard Shantsila, Gregory Y H Lip
Nov 13, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Daniel YacoubYahye Merhi
May 7, 2011·Vox Sanguinis·M EtscheidJ Dodt
Mar 28, 2012·Transfusion·Gregory W DanielHector S Izurieta
Jun 5, 2012·Journal of Atherosclerosis and Thrombosis·Tatsuya IshikawaShigeharu Takagi
Nov 1, 2012·Transfusion·Douglass A DrelichJay H Herman
Citations
Jul 23, 2015·Vox Sanguinis·C F YuJ Z Wang
Aug 7, 2014·Biologicals : Journal of the International Association of Biological Standardization·W A GermishuizenT Burnouf
Dec 20, 2018·Immunologic Research·Merav LidarYair Levy