PMID: 7518528Aug 1, 1994Paper

Significance of the immune response to a major, conformational B-cell epitope on the hepatitis C virus NS3 region defined by a human monoclonal antibody

Journal of Virology
M U MondelliW Habets

Abstract

The nonstructural protein NS3 of hepatitis C virus (HCV) possesses two enzymatic domains which are thought to be essential for the virus life cycle: an N-terminal serine-type proteinase, responsible for the processing of nonstructural polypeptides, and a C-terminal nucleoside triphosphatase/helicase, presumably involved in the unwinding of the viral genome. The human antibody response to NS3 usually appears early in the course of HCV infection and is predominantly directed against the carboxyl-terminal portion; however, its fine specificity and clinical significance are largely unknown. We have generated a human monoclonal antibody (hMAb), designated CM3.B6, from a cloned B-cell line obtained from the peripheral blood of a patient with chronic HCV infection, which selectively recognized the purified NS3 protein expressed in bacteria or in eukaryotic cells transfected with full-length or NS3 cDNA. Fine-specificity studies revealed that CM3.B6 recognized a 92-amino-acid sequence (clone 8, amino acids 1363 to 1454) selected from an NS3 DNase fragment library but failed to bind to 12-mer peptides synthesized from the same region, suggesting recognition of a conformational B-cell epitope. Experiments using deletion mutants of clone ...Continue Reading

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Citations

Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A CerinoM U Mondelli
Jan 30, 2004·Journal of Medical Virology·Colette Jolivet-ReynaudDominique Rolland
Mar 21, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Stefania VarchettaMario U Mondelli
Sep 15, 2005·The Journal of Experimental Medicine·Daniele AccapezzatoVincenzo Barnaba
Jun 23, 2015·Immunology·Stefania MantovaniMario U Mondelli
Aug 1, 1995·European Journal of Clinical Investigation·M U MondelliA Cerino
Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M ChenD R Milich
Oct 15, 2019·Frontiers in Immunology·Antonella CerinoMario U Mondelli

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