PMID: 45548Jan 1, 1979

Significance of the variation in isozymes of liver lactate dehydrogenase with thermal acclimation in goldfish--II. Effect of pH

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
H Yamawaki, H Tsukuda

Abstract

1. Effect of pH on liver lactate dehydrogenase (LDH) and its isozymes was examined in the goldfish acclimated to different temperatures and some purification of the LDH was attempted. 2. The optimal pH and the Km value at 30 degrees C of the enzyme were independent of acclimation temperature. 3. the optimal pH of isozyme was more basic in the order of LDH-1, LDH-2, LDH-3, LDH-4 and LDH-5. Km values of isozymes at 30 degrees C were higher in the order of LDH-1, LDH-3 and LDH-5. 4. There was no change in the enzyme activity during thermal acclimation.

References

Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H Yamawaki, H Tsukuda
Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K Tsugawa
Nov 15, 1975·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H Tsukuda
Oct 1, 1972·The Journal of Experimental Zoology·D H Whitmore, E Goldberg
Sep 15, 1971·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T W Moon, P W Hochachka
Jul 1, 1972·Comparative Biochemistry and Physiology. A, Comparative Physiology·S C Cooper, J H Ferguson
Jul 14, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·J F Burd, M Usategui-Gomez
Aug 15, 1971·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P W Hochachka, J K Lewis
Dec 1, 1968·Comparative Biochemistry and Physiology·P W Hochachka, G N Somero

Citations

Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M L Schwantes, A R Schwantes
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Z L CoppesA R Schwantes
Jan 1, 1990·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·T O SasidharanP K Ghosh

Related Concepts

Acclimatization
Dace
Goldfish
Hydrogen-Ion Concentration
Alloenzymes
Lactate Dehydrogenase
Liver

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