Significant contamination of superoxide dismutases and catalases with lipopolysaccharide-like substances

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Yoshihisa KubotaHiroshi Sato

Abstract

Commercially available superoxide dismutase (SOD) and catalase induced remarkable morphological changes in cultured peritoneal resident macrophages (PRMs). The morphology resembled that of cells stimulated with various macrophage-activating substances such as bacterial lipopolysaccharides (LPS). PRMs treated with SOD and catalase for 24 h produced a significant amount of nitric oxide. Peritoneal thioglycollate-exudated macrophages (PEMs) that were induced to die by combined treatment with LPS and cycloheximide (protein synthesis inhibitor) were killed by the treatment with SOD and catalase in the presence of cycloheximide. The effect of SOD and catalase was heat-resistant and was not found in PEMs of the C3H/HeJ mouse, an LPS low responder strain. These results strongly indicated the presence of LPS-like substances in the sample of SOD and catalase used. An examination of commercially available SOD and catalase samples for the presence of LPS-like substances demonstrated that many were contaminated. The LPS contamination was directly confirmed by bacterial endotixins test. It is quite important to take into consideration that commercially available SODs and catalases may be contaminated with LPS and the contaminants may affect ...Continue Reading

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Citations

Dec 30, 2008·Free Radical Biology & Medicine·Barry Halliwell
Jun 15, 2018·Molecular Medicine Reports·Hu-Nan SunYing-Hao Han
Sep 9, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Luís F GriloSusana P Pereira

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