Abstract
Numerous adults' studies demonstrated that preaphaeresis CD34+ cells significantly correlate with the number of CD34+ cells collected by the aphaeresis procedure. Equivalent studies in children are scarce. We studied retrospectively 92 aphaeresis procedures performed following chemotherapy (44) or in steady state (48) in 60 pediatric patients (40 males, 20 females), median age of 7.5 years. Aphaeresis procedures were performed using a SPECTRA Optica (TERUMOBCT) continuous flow cell separator. CD34+ cell concentrations were assessed using flow cytometry. A highly significant correlation between peripheral CD34 cell count on the day of aphaeresis and CD34 cell yield per kg (R2 = .824, P < .0001) was demonstrated. A higher preaphaeresis CD34 cell count was demonstrated in patients with higher preaphaeresis white blood cell count, in patients with brain tumors, and in patients who received chemotherapy as part of their mobilization protocol. A threshold number of 20 peripheral CD34+ cell/μL was found to predict harvesting of 3 × 106 stem cells/kg, and 30 peripheral CD34+ cell/μL for harvesting of 5 × 106 stem cells/kg. This significant correlation between peripheral CD34 cell count and CD34 cell yield, and the threshold number of ...Continue Reading
References
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C A Uyl-de GrootF F Rutten
Jun 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J BeyerW Stieger
Oct 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·W BensingerC Weaver
Feb 2, 1997·Bone Marrow Transplantation·J E KissE D Ball
Oct 23, 1997·Bone Marrow Transplantation·S ArmitageC Navarrete
May 20, 1998·Medical and Pediatric Oncology·S S GoldbergK K Matthay
May 22, 1998·Transfusion·J A Pérez-SimónJ F San Miguel
May 21, 1999·Transfusion·J YuS D Rowley
Sep 22, 1999·Bone Marrow Transplantation·J PerentesisN Ramsay
Jul 16, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Andrew D CampbellMorris Kletzel
Aug 18, 2005·Journal of Clinical Apheresis·A L BasquieraJ J García
Nov 8, 2005·Bone Marrow Transplantation·C J FraserM R Verneris
Aug 22, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Odile OberlinUNKNOWN Société Française des Cancers de l'Enfant
Oct 3, 2006·The Lancet Oncology·Amar GajjarRichard J Gilbertson
Nov 26, 2008·Bone Marrow Transplantation·A ClaviezN Schmitz
Jul 20, 2010·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Richard E HarrisMitchell S Cairo
Jan 1, 2007·Asian Journal of Transfusion Science·Aleksandar Mijovic, Derwood Pamphilon
Oct 11, 2011·Transfusion·Luca PierelliUNKNOWN Gruppo Italiano Trapianto Midollo Osseo (GITMO)
Jan 10, 2012·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Peter GambellH Miles Prince
Mar 17, 2012·Cytotherapy·Piya RujkijyanontWing Leung
Oct 5, 2012·Transfusion·Simone CesaroArcangelo Prete
Oct 1, 2013·Bone Marrow Transplantation·Z ShuD Gao
Jun 24, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Hakan GokerYahya Buyukasik
Apr 5, 2016·Bone Marrow Transplantation·S Proust-HoudemontD Valteau-Couanet