Significant correlation between refractive index and activity of mitochondria: single mitochondrion study

Biomedical Optics Express
Keisuke HasedaYoshihiro Ohta

Abstract

Measurements of refractive indices (RIs) of intracellular components can provide useful information on the structure and function of cells. The present study reports, for the first time, determination of the RI of an isolated mitochondrion in isotonic solution using retardation-modulated differential interference contrast microscopy. The value was 1.41 ± 0.01, indicating that mitochondria are densely packed with molecules having high RIs. Further, the RIs of each mitochondrion were significantly correlated with the mitochondrial membrane potential, an index of mitochondrial activity. These results will provide useful information on the structures and functions of cells based on the intracellular distribution of RIs.

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Citations

Jun 4, 2015·Biochemical and Biophysical Research Communications·Takahiro ShibataYoshihiro Ohta
Nov 14, 2015·Journal of Biophotonics·Daniel M de BruinDirk J Faber
Nov 24, 2017·Scientific Reports·Kotoe HirusakiYoshihiro Ohta
Jul 10, 2019·Biotechnology and Bioengineering·Elisabete C CostaIlídio J Correia
Aug 21, 2019·Micromachines·Qian FengBenoît Kornmann
Jun 22, 2017·Therapeutic Delivery·Renat R Letfullin, Scott A Szatkowski
Jul 14, 2018·Scientific Reports·Z ZhangT Miyake
Jul 22, 2019·Scientific Reports·Vinoth BalasubramaniChau-Jern Cheng
Apr 18, 2018·Analytical and Bioanalytical Chemistry·Katherine A MuratoreEdgar A Arriaga
May 8, 2018·Chemical Science·Jin-Sung ParkMinhaeng Cho
Nov 15, 2020·Photodiagnosis and Photodynamic Therapy·Banat GulIftikhar Ahmad
Apr 15, 2021·Plant Biotechnology·Yoshinori AbeMasatsugu Toyota
Apr 26, 2021·Trends in Biotechnology·Kelvin C M LeeKevin K Tsia
May 19, 2021·Translational Vision Science & Technology·Tingwei ZhangVivek J Srinivasan
Jun 12, 2021·Experimental Biology and Medicine·Xincheng YaoDavid Le
Sep 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fabio Alejandro Aguilar MoraHan Moshage

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