Significant gene expression differences in histologically "Normal" liver biopsies: Implications for control tissue.

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Tarik AsselahMichel Vidaud

Abstract

Gene expression technologies allow the analysis of gene networks whose expression is associated with specific pathological conditions compared with normal tissue. We hypothesized that histologically normal tissue obtained in different ways (percutaneous or surgical liver biopsies), usually used as normal controls in gene expression studies, could have different gene expression patterns. Group A comprised percutaneous liver biopsies in 14 patients with mildly elevated alanine aminotransferase in whom all causes of liver disease had been ruled out. Group B comprised 14 surgical liver biopsies of nontumoral livers. All 28 specimens were histologically normal. Real-time quantitative reverse-transcription polymerase chain reaction were used to compare the messenger RNA expression of 240 selected genes in these two groups. Expression of 26 of the 240 genes was significantly different between groups A and B; 23 genes were up-regulated in group A, while three were down-regulated in group B. The most notable changes occurred in the inflammatory response family genes. Eight genes discriminated perfectly between groups A and B: seven up-regulated genes (PAI1, THBS1, IL8, PTGS2, CXCR4, JUN, and FOS), and one down-regulated gene (IHH). In c...Continue Reading

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Citations

Nov 16, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Tarik Asselah
Feb 25, 2010·Current Opinion in Gastroenterology·Keyur Patel
Dec 17, 2008·Gut·T AsselahP Marcellin
Mar 29, 2014·Current Opinion in Gastroenterology·Keyur Patel, Nicholas A Shackel
Jun 9, 2010·The Journal of Pathology·Tarik AsselahRichard Moreau
Sep 15, 2011·Alcoholism, Clinical and Experimental Research·Hédia BoujedidiSylvie Naveau
Mar 18, 2009·Human Pathology·Jay H Lefkowitch
Jan 13, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bianca M ArendtJohane P Allard
Oct 1, 2013·International Journal of Endocrinology·Elisabetta Bacchi, Paolo Moghetti
Oct 3, 2012·PPAR Research·Valerio PazienzaGianluigi Mazzoccoli
Feb 6, 2009·Expert Review of Anticancer Therapy·Gerhard Treiber

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Methods Mentioned

BETA
biopsies
biopsy
PCR
electrophoresis

Software Mentioned

PE Biosystems
Oligo

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