Significantly enhanced production of acarbose in fed-batch fermentation with the addition of S-adenosylmethionine

Journal of Microbiology and Biotechnology
Li-Hui SunYu-Guo Zheng

Abstract

Acarbose, a pseudo-oligosaccharide, is widely used clinically in therapies for non-insulin-dependent diabetes. In the present study, S-adenosylmethionine (SAM) was added to selected media in order to investigate its effect on acarbose fermentation by Actinoplanes utahensis ZJB- 08196. Acarbose titer was seen to increase markedly when concentrations of SAM were added over a period of time. The effects of glucose and maltose on the production of acarbose were investigated in both batch and fed-batch fermentation. Optimal acarbose production was observed at relatively low glucose levels and high maltose levels. Based on these results, a further fed-batch experiment was designed so as to enhance the production of acarbose. Fed-batch fermentation was carried out at an initial glucose level of 10 g/l and an initial maltose level of 60 g/l. Then, 12 h post inoculation, 100 micromol/l SAM was added. In addition, 8 g/l of glucose was added every 24 h, and 20 g/l of maltose was added at 96 h. By way of this novel feeding strategy, the maximum titer of acarbose achieved was 6,113 mg/l at 192 h. To our knowledge, the production level of acarbose achieved in this study is the highest ever reported.

Citations

Nov 13, 2012·Applied Microbiology and Biotechnology·Kozo Ochi, Takeshi Hosaka
Aug 30, 2014·Journal of Biotechnology·Sergej WendlerAlfred Pühler
Mar 21, 2020·Nature Communications·Qinqin ZhaoZixin Deng

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