Significantly improved accuracy of diagnosis of early Lyme disease by peptide enzyme-linked immunosorbent assay based on the borreliacidal antibody epitope of Borrelia burgdorferi OspC.

Clinical and Vaccine Immunology : CVI
Dean A JobeSteven M Callister

Abstract

Highly specific borreliacidal antibodies are induced by infection with Borrelia burgdorferi, and the immunodominant response during early Lyme disease is specific for an epitope within the 7 amino acids nearest the C terminus of OspC. We evaluated the ability of an enzyme-linked immunosorbent assay (ELISA) based on a synthetic peptide (OspC7) that matched the region to detect the response and compared the sensitivity during early Lyme disease to that for an FDA-approved Western blot. When the optical density value was adjusted to 98% specificity based on the results from testing normal or uncharacterized sera (n = 236) or sera from patients with blood factors or illnesses that commonly produce antibodies that cross-react with B. burgdorferi antigens (n = 77), 115 (73%) of 157 sera from patients likely to have early Lyme disease were positive for immunoglobulin M (IgM) antibodies and 17 (11%) also had IgG antibodies. In addition, the IgM ELISA reactivities and the titers of antibodies detected by a flow cytometric borreliacidal antibody test correlated closely (r = 0.646). Moreover, the IgM ELISA was significantly more sensitive (P < 0.001) than the Western blot procedure. The findings therefore confirmed that the peptide IgM EL...Continue Reading

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Citations

Apr 22, 2011·Clinical and Vaccine Immunology : CVI·Dean A JobeSteven M Callister
Jun 22, 2012·Clinical and Vaccine Immunology : CVI·Monica E EmbersMario T Philipp
Jul 7, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Larisa IvanovaMaria Gomes-Solecki
Mar 5, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Steven M CallisterPaul M Arnaboldi
Oct 16, 2012·Diagnostic Microbiology and Infectious Disease·Gary P WormserJ Stephen Dumler
Jun 13, 2015·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Steven M CallisterTerri L Wasmoen
Jul 23, 2011·Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases·Leonard H Sigal
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Caoimhe RobinsonAlan O'Riordan
Oct 17, 2019·Journal of Proteome Research·Yong ZhouLeroy Hood

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