Silencing MIG1 in Saccharomyces cerevisiae: effects of antisense MIG1 expression and MIG1 gene disruption.

Applied and Environmental Microbiology
Lisbeth OlssonJens Nielsen

Abstract

Silencing of MIG1, a transcription factor imposing carbon catabolite repression on invertase, was attempted, either by disrupting the gene or by expressing antisense copies of the gene. The performance of the recombinant strains in bioreactor batch cultivations on sucrose, in the presence of glucose, was compared with that of the wild-type strain under the same conditions. In the delta migI strain, the rate of sucrose utilization was independent (10 mmol/g/h) of the glucose concentration. During the cultivations with the wild-type strain and the antisense strains, two distinct phases were observed. The rates of sucrose hydrolysis were < 1 mmol/g/h and 9 to 10 mmol/g/h in the first and second phases, respectively. Entry into the second cultivation phase was characterized by a decline in glucose concentration below 12 mmol/liter. As expected, disruption of MIG1 resulted in a relief of glucose repression. However, silencing of MIG1 expression was not achieved by expressing antisense MIG1, even though antisense MIG1 RNA was sufficiently stable to be detected. In the wild-type and delta migI strains, the specific growth rate was 0.32 to 0.33 h-1, whereas it was lower in the antisense strains, 0.25 to 0.30 h-1.

References

Mar 2, 1977·Biochimica Et Biophysica Acta·M V ElorzaR Sentandreu
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K StruhlR W Davis
Jan 1, 1992·Molecular Microbiology·R J Trumbly
Jun 1, 1992·Applied Microbiology and Biotechnology·B Knust, D von Wettstein
Jun 1, 1992·European Journal of Biochemistry·J M Gancedo
Feb 21, 1992·Cell·C A KeleherA D Johnson
Mar 1, 1990·Journal of General Microbiology·C VerduynJ P van Dijken
May 25, 1990·Nucleic Acids Research·M E SchmittB L Trumpower
Nov 1, 1987·Journal of Bacteriology·M Carlson
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Jul 1, 1982·Archives of Biochemistry and Biophysics·E SantosR Sentandreu
Sep 1, 1996·Molecular and Cellular Biology·L L Lutfiyya, M Johnston
Jun 5, 1992·Biotechnology and Bioengineering·A Patkar, J H Seo

❮ Previous
Next ❯

Citations

Dec 23, 2011·FEMS Yeast Research·Il-Kwon KimJens Nielsen
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·S OstergaardJ Nielsen
Feb 19, 1998·Microbiology·Christopher J L KleinJens Nielsen
Oct 23, 2001·Biotechnology and Bioengineering·C HerwigU von Stockar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.