Silencing of Barkor/ATG14 sensitizes osteosarcoma cells to cisplatin‑induced apoptosis

International Journal of Molecular Medicine
Zhifang ZhaoHuimin Tao

Abstract

Although surgical excision following neoadjuvant chemotherapy has contributed to the long-term survival of osteosarcoma patients, patients that do not respond to commonly used drugs including cisplatin, have a poor prognosis. Autophagy is important in the inhibition of chemotherapeutic apoptosis. Therefore, we investigated whether knockdown of Beclin1-associated autophagy-related key regulator (Barkor/ATG14) promoted cisplatin-induced apoptosis in a drug-resistant osteosarcoma cell line in vitro. Saos-2 cells were transfected with Barkor siRNA. Sensitivity of the Barkor siRNA-transfected cell line to cisplatin was evaluated. Silencing of Barkor did not directly inhibit the growth rate of the transfected cells, but it significantly increased their sensitivity to cisplatin. The results of flow cytometry and 4',6-diamidino-2-phenylindole (DAPI) staining revealed that Barkor siRNA-transfected Saos-2 cells treated with cisplatin exhibited much higher rates of apoptosis than the control and control siRNA-transfected cells. Additionally, the combination of silencing of Barkor with cisplatin treatment promoted the expression of caspase-12 and calpain. The increase of cisplatin cytotoxicity may therefore be involved in endoplasmic retic...Continue Reading

References

Jul 13, 2001·The Journal of Biological Chemistry·R V RaoD E Bredesen
Nov 29, 2002·Eukaryotic Cell·Fulvio Reggiori, Daniel J Klionsky
Sep 15, 2004·Journal of Chemical Neuroanatomy·Takashi Momoi
Mar 19, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paul A MeyersHolcombe Grier
Oct 4, 2005·The Journal of Clinical Investigation·Beth Levine, Junying Yuan
Oct 26, 2005·Cell Death and Differentiation·P Codogno, A J Meijer
Jan 25, 2007·Orphanet Journal of Rare Diseases·Piero Picci
Oct 10, 2008·Molecular Biology of the Cell·Eisuke ItakuraNoboru Mizushima
Dec 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qiming SunQing Zhong
Mar 10, 2009·Nature Cell Biology·Kohichi MatsunagaTamotsu Yoshimori
Mar 10, 2010·Cancer Treatment and Research·Giulia Ottaviani, Norman Jaffe
Jul 20, 2010·Apoptosis : an International Journal on Programmed Cell Death·Juan A MartinezStephen F Larner
Sep 3, 2010·Nature Cell Biology·Zhifen Yang, Daniel J Klionsky
Apr 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Weiliang FanQing Zhong
Nov 22, 2011·Cancer Research·Jun HuangDaolin Tang

❮ Previous
Next ❯

Citations

Jan 11, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiu-Guo HanTing-Ting Tang
Mar 26, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chaoqun YangXuhui Zhou
Feb 10, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Alessandra ChesiStruan Fa Grant
Apr 26, 2017·Veterinary Pathology·M MassiminiL Della Salda
Mar 30, 2017·Experimental and Therapeutic Medicine·Hongyan LiuShu Wang
Jul 18, 2018·International Journal of Molecular Medicine·Cuiyun LiGuilong Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
fluorescence microscopy
scraping
protein assay
PCR

Related Concepts

Related Feeds

ATG proteins

The discovery of autophagy-related ('ATG') proteins in the 1990s greatly advanced the mechanistic understanding of autophagy and clarified the fact that autophagy serves important roles in various biological processes.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms