Silencing of circFoxO3 Protects HT22 Cells from Glutamate-Induced Oxidative Injury via Regulating the Mitochondrial Apoptosis Pathway.

Cellular and Molecular Neurobiology
Shao-Peng LinXiao-Hui Chen

Abstract

Recent studies demonstrated that FoxO3 circular RNA (circFoxO3) plays an important regulatory role in tumourigenesis and cardiomyopathy. However, the role of circFoxO3 in neurodegenerative diseases remains unknown. The aim of this study was to examine the possible role of circFoxO3 in neurodegenerative diseases and the underlying mechanisms. To model human neurodegenerative conditions, hippocampus-derived neurons were treated with glutamate. Using molecular and cellular biology approaches, we found that circFoxO3 expression was significantly higher in the glutamate treatment group than that in the control group. Furthermore, silencing of circFoxO3 protected HT22 cells from glutamate-induced oxidative injury through the inhibition of the mitochondrial apoptotic pathway. Collectively, our study demonstrates that endogenous circFoxO3 plays a key role in inducing apoptosis and neuronal cell death and may act as a novel therapeutic target for neurodegenerative diseases.

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Citations

Jan 30, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Rares DrulaIoana Berindan-Neagoe
Feb 23, 2021·Molecular Therapy. Nucleic Acids·Lei ZhangPeifeng Li
Apr 10, 2021·Frontiers in Genetics·Tianli YangRuipeng Jia
Jun 22, 2021·Frontiers in Cell and Developmental Biology·Dean RaoWenjie Huang
Jul 15, 2021·Oxidative Medicine and Cellular Longevity·Yunguang WangJuan Jin
Aug 18, 2021·Journal of Chemical Neuroanatomy·Vaibhav OliPravir Kumar
Aug 28, 2021·Molecular Medicine·Mei TaoShaoqing Ju

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Methods Mentioned

BETA
PCR
fluorescence microscopy
transfection
fluorescence
nuclear translocation

Software Mentioned

Pro Plus
Graph Pad Prism
Image

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