Silencing of either SR-A or CD36 reduces atherosclerosis in hyperlipidaemic mice and reveals reciprocal upregulation of these receptors

Cardiovascular Research
Petri I MäkinenSeppo Ylä-Herttuala

Abstract

Macrophage scavenger receptor A (SR-A) and class B scavenger receptor CD36 (CD36) contribute to foam cell formation and atherogenesis via uptake of modified lipoproteins. So far, the role of these scavenger receptors has been studied mainly using knockout models totally lacking these receptors. We studied the role of SR-A and CD36 in foam cell formation and atherogenesis by RNA interference (RNAi)-mediated silencing, which is a clinically feasible method to down-regulate the expression of these receptors. We constructed lentivirus vectors encoding short hairpin RNAs (shRNAs) against mouse SR-A and CD36. Decreased SR-A but not CD36 expression led to reduced foam cell formation caused by acetylated low-density lipoprotein (LDL) in mouse macrophages, whereas the uptake of oxidized LDL was not altered. More importantly, silencing of SR-A upregulates CD36 and vice versa. In LDL receptor-deficient apolipoprotein B100 (LDLR(-/-)ApoB(100/100)) mice kept on a western diet, silencing of either SR-A or CD36 in bone marrow cells led to a marked decrease (37.4 and 34.2%, respectively) in cross-sectional lesion area, whereas simultaneous silencing of both receptors was not effective. Our results suggest that silencing of either SR-A or CD36 ...Continue Reading

References

Apr 6, 1989·The New England Journal of Medicine·D SteinbergJ L Witztum
Oct 1, 1989·The Journal of Clinical Investigation·S Ylä-HerttualaD Steinberg
Jul 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S Ylä-HerttualaT Nikkari
May 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·K J Williams, I Tabas
Jun 6, 1998·Zeitschrift für Orthopädie und ihre Grenzgebiete·W SchneiderK Knahr
Oct 28, 1998·The Journal of Clinical Investigation·M M VéniantS G Young
Dec 16, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·V R BabaevM F Linton
Jan 3, 2001·Nature Medicine·K J MooreM W Freeman
Nov 2, 2002·Nature Medicine·Andrew C Li, Christopher K Glass
Dec 11, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Johanna JalkanenSeppo Ylä-Herttuala
Oct 16, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Maria FebbraioRoy L Silverstein
Aug 3, 2005·The Journal of Clinical Investigation·Joseph L Witztum
Jan 4, 2006·The Journal of Gene Medicine·Petri I MäkinenSeppo Ylä-Herttuala
Jul 1, 2008·Archives of Biochemistry and Biophysics·Atsushi MaruyamaKen Itoh
Oct 25, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Jennifer J Manning-TobinMason W Freeman

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Citations

Dec 17, 2010·Biogerontology·Timo E Strandberg, Sarianna Sipilä
Jan 31, 2012·Current Atherosclerosis Reports·Tanu GoyalJawahar L Mehta
Oct 12, 2010·Cardiovascular Research·Toshihiro Tsuruda
Jun 13, 2012·Cardiovascular Research·Chin-Sheng LinJenn-Haung Lai
May 12, 2012·Mediators of Inflammation·Ting GuiYasuteru Muragaki
Mar 17, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Allison B Reiss, Bruce N Cronstein
May 21, 2013·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Justin W CaravaggioStewart C Whitman
Oct 24, 2015·Journal of Cellular and Molecular Medicine·Dimitry A ChistiakovAlexander N Orekhov
Mar 23, 2012·Immunobiology·Julia KzhyshkowskaSiamon Gordon
Jun 24, 2015·Cellular and Molecular Life Sciences : CMLS·Dennis WolfKlaus Ley
Jun 21, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xiao-Hua YuChao-Ke Tang
Jun 27, 2013·International Reviews of Immunology·Ana B SanzAlberto Ortiz
Jul 26, 2011·International Immunopharmacology·Hong YangYa-lei Dai
Dec 24, 2013·Biochemical and Biophysical Research Communications·Ha Young LeeYoe-Sik Bae
Jun 4, 2016·Vascular Pharmacology·Natalia Di PietroAssunta Pandolfi
Aug 11, 2016·Frontiers in Immunology·Zarah BatulanEdward R O'Brien
Apr 4, 2017·Journal of Agricultural and Food Chemistry·Sy-Jou ChenChin-Sheng Lin
Apr 9, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Seppo Ylä-Herttuala, Andrew H Baker
May 27, 2015·Cells·Izma Abdul ZaniSreenivasan Ponnambalam
Mar 13, 2012·Biomedical Chromatography : BMC·Yun LingChenggang Huang
Aug 1, 2013·Physiological Reviews·Paul N Hopkins
Sep 3, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Naimeh RafatianStewart C Whitman
Oct 28, 2019·International Journal of Molecular Sciences·Beatriz Herrero-FernandezJose Maria Gonzalez-Granado
Jan 15, 2013·Current Opinion in Lipidology·Petri I Mäkinen, Seppo Ylä-Herttuala
Jul 14, 2020·Immune Network·Jihye Lee, Jae-Hoon Choi
Aug 9, 2017·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Dimitry A ChistiakovAlexander N Orekhov
Aug 1, 2020·BioMed Research International·Manman DouYingqi Xing
Apr 16, 2016·Molecular Medicine Reports·Janeesh Plakkal AyyappanYoung-Hwa Goo
Sep 27, 2019·Pharmacological Reviews·Dongdong WangAtanas G Atanasov
Jan 12, 2019·Frontiers in Immunology·Daniel A Patten, Shishir Shetty

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