Silencing of endogenous envelope genes in human choriocarcinoma cells shows that envPb1 is involved in heterotypic cell fusions.

The Journal of General Virology
Lars AagaardJohn J Rossi

Abstract

Syncytin-1 and envPb1 are two conserved envelope genes in the human genome encoded by single loci from the HERV-W and -Pb families, respectively. To characterize the role of these envelope proteins in cell-cell fusion, we have developed lentiviral vectors that express short hairpin RNAs for stable knockdown of syncytin-1 and envPb1. Analysis of heterotypic fusion activity between trophoblast-derived choriocarcinoma BeWo cells, in which syncytin-1 and envPb1 are specifically silenced, and endothelial cells demonstrated that both syncytin-1 and envPb1 are important to fusion. The ability to fuse cells makes syncytin-1 and envPb1 attractive candidate molecules in therapy against cancer. Our available vectors may help eventually to decipher roles for these genes in human health and/or disease.

References

Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Oct 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sandra BlaiseThierry Heidmann
Aug 19, 2004·Cellular and Molecular Life Sciences : CMLS·K MortensenL-I Larsson
Oct 13, 2004·Retrovirology·Palle VillesenFinn Skou Pedersen
Aug 12, 2005·Cytogenetic and Genome Research·S PrudhommeF Mallet
Jul 18, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dong Sung AnIrvin S Y Chen
Jul 28, 2006·Cellular and Molecular Life Sciences : CMLS·B BjerregaardL-I Larsson
Oct 27, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Reiner StrickPamela L Strissel
Nov 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Cécile EsnaultThierry Heidmann
Mar 31, 2009·Cancer Letters·Julie Mou LarsenLars-Inge Larsson
Oct 9, 2009·Nature·Donald F ConradMatthew E Hurles
Jul 29, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Matthias RuebnerReiner Strick

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Citations

Jun 7, 2014·Cell and Tissue Research·Bolette BjerregardLars-Inge Larsson
Dec 31, 2019·The Journal of Investigative Dermatology·Kirsi LaukkanenAnnamari Ranki

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