Silencing of G0/G1 switch gene 2 in cutaneous squamous cell carcinoma

PloS One
Yoshimasa NobeyamaHidemi Nakagawa

Abstract

Methylation of a CpG island (CGI; a dense cluster of CpGs) located in the 5' region of a gene suppresses that gene's transcription. The expression of G0/G1 switch gene 2 (G0S2) is potentially associated with tumorigenesis. The aim of this study is to elucidate the methylation status of the CGI located in the 5' region of G0S2 (hereinafter called 5' G0S2 CGI) in cutaneous squamous cell carcinoma (SCC). Quantitative real-time methylation-specific PCR (RT-MSP) and bisulfite sequencing were performed to evaluate the methylation statuses of cutaneous SCC and normal epithelial cell samples. Quantitative real-time reverse transcription-PCR was performed to evaluate RNA expression levels. Immunohistochemical analysis was performed to detect protein expression. G0S2 was suppressed in the five SCC cell lines with 5' G0S2 CGI methylation levels of nearly 100.0% and was expressed in the two normal cultured keratinocytes with methylation levels of almost 0.0%. G0S2 was re-expressed in SCC cell lines treated with a demethylating agent. The in vivo methylation levels of 5' G0S2 CGI as determined by RT-MSP varied widely (0.0% to 77.7%) in 17 cutaneous SCC samples and narrowly (0.1% to 7.3%) in 6 normal epidermis samples. Nine cutaneous SCC sam...Continue Reading

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Citations

Sep 10, 2020·Journal of Clinical Medicine·Taxiarchis Konstantinos NikolouzakisJohn Tsiaoussis
Oct 27, 2020·Journal of Toxicology and Environmental Health. Part a·Min-Kyung JooYoung-Jin Chun
Jun 12, 2018·Pharmacology & Therapeutics·Melina MitsiogianniMihalis I Panayiotidis
Jul 25, 2021·Cancers·Eun Kyung Ko, Brian C Capell

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Methods Mentioned

BETA
PCR
reverse transcription-PCR

Software Mentioned

SPSS

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