Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27

Genes & Development
Antonis KirmizisPeggy J Farnham

Abstract

Polycomb group (PcG) complexes 2 and 3 are involved in transcriptional silencing. These complexes contain a histone lysine methyltransferase (HKMT) activity that targets different lysine residues on histones H1 or H3 in vitro. However, it is not known if these histones are methylation targets in vivo because the human PRC2/3 complexes have not been studied in the context of a natural promoter because of the lack of known target genes. Here we report the use of RNA expression arrays and CpG-island DNA arrays to identify and characterize human PRC2/3 target genes. Using oligonucleotide arrays, we first identified a cohort of genes whose expression changes upon siRNA-mediated removal of Suz12, a core component of PRC2/3, from colon cancer cells. To determine which of the putative target genes are directly bound by Suz12 and to precisely map the binding of Suz12 to those promoters, we combined a high-resolution chromatin immunoprecipitation (ChIP) analysis with custom oligonucleotide promoter arrays. We next identified additional putative Suz12 target genes by using ChIP coupled to CpG-island microarrays. We showed that HKMT-Ezh2 and Eed, two other components of the PRC2/3 complexes, colocalize to the target promoters with Suz12. I...Continue Reading

References

Aug 10, 1999·Seminars in Cell & Developmental Biology·J J Jacobs, M van Lohuizen
Nov 1, 2000·Cell·M Bienz, H Clevers
Mar 16, 2001·Current Opinion in Genetics & Development·H W Brock, M van Lohuizen
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·J I KoontzJ Sklar
Sep 21, 2001·Molecular and Cellular Biology·A S WeinmannP J Farnham
May 22, 2002·Biochimica Et Biophysica Acta·Jacqueline J L Jacobs, Maarten van Lohuizen
Oct 11, 2002·Nature·Sooryanarayana VaramballyArul M Chinnaiyan
Feb 8, 2003·Current Biology : CB·Jeffrey A Simon
Mar 12, 2003·Cell·Valerio Orlando
Apr 17, 2003·Molecular and Cellular Biology·Feng TiePeter J Harte
May 2, 2003·Genetica·Vincenzo PirrottaMaxim Pilyugin
Aug 12, 2003·The Journal of Biological Chemistry·Matthew J OberleyPeggy J Farnham
Sep 23, 2003·Developmental Biology·Sébastien BloyerJean-Maurice Dura
Sep 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Celina G KleerArul M Chinnaiyan
Jan 31, 2004·Cell Cycle·Diego PasiniKristian Helin
Feb 21, 2004·Methods in Enzymology·Matthew J OberleyPeggy J Farnham
Apr 1, 2004·Molecular Cell·Gaetano I DellinoVincenzo Pirrotta
Dec 19, 2006·Nature Reviews. Genetics·Yuri B Schwartz, Vincenzo Pirrotta

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Citations

Feb 2, 2013·Human Genetics·María Berdasco, Manel Esteller
Nov 16, 2007·Cell and Tissue Research·Stuart Atkinson, Lyle Armstrong
Feb 17, 2010·Journal of Mammary Gland Biology and Neoplasia·Monique RijnkelsEve Devinoy
Feb 7, 2013·Journal of Biosciences·Kwanghyun LeeBong-Gun Ju
Feb 16, 2011·Cell Research·Hsing-Chen Tsai, Stephen B Baylin
Dec 17, 2005·Nature·Emmanuelle ViréFrançois Fuks
Sep 13, 2005·Nature Genetics·Yoshiko MitoSteven Henikoff
Nov 13, 2013·Nature Reviews. Clinical Oncology·Mark RoschewskiWyndham H Wilson
Oct 20, 2011·Nature Reviews. Gastroenterology & Hepatology·Victoria Valinluck Lao, William M Grady
Sep 10, 2009·Nature Reviews. Molecular Cell Biology·Jeffrey A Simon, Robert E Kingston
Sep 8, 2009·Nature Structural & Molecular Biology·Marcus BuschbeckLuciano Di Croce
Jun 5, 2012·Nature Structural & Molecular Biology·Sònia GuilManel Esteller
Nov 13, 2012·Nature Structural & Molecular Biology·Catherine A MusselmanTatiana G Kutateladze
Feb 8, 2006·Nature Structural & Molecular Biology·Marc DamelinTimothy H Bestor
Aug 29, 2006·Nature Structural & Molecular Biology·Daniel H KimJohn J Rossi
Feb 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alexandros TzatsosPhilip N Tsichlis
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lifeng WangKai Ge
Nov 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christopher J SneeringerRobert A Copeland
Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kirsty JamiesonEric U Selker
Apr 24, 2008·The Journal of Biological Chemistry·Satoshi FujiiAtsushi Ochiai
Feb 16, 2013·Assay and Drug Development Technologies·Jeffrey R SimardVioleta Yu
Feb 9, 2005·DNA and Cell Biology·Jesús GilGordon Peters
Mar 24, 2009·Nucleic Acids Research·Jan LukasVladimir Korinek
Mar 15, 2012·Nucleic Acids Research·Jia ZengJianpeng Ma
Nov 3, 2004·Genes & Development·Giuseppina CarettiVittorio Sartorelli
Jun 21, 2005·Genes & Development·Sophia W M BruggemanMaarten van Lohuizen

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