Silencing of LncRNA-HOTAIR decreases drug resistance of Non-Small Cell Lung Cancer cells by inactivating autophagy via suppressing the phosphorylation of ULK1

Biochemical and Biophysical Research Communications
Yan YangJun Zou

Abstract

Drug resistance is an important factor leading to the recurrence and metastasis of Non-Small Cell Lung Cancer (NSCLC). Long non-coding RNAs (LncRNAs) play important roles in drug resistance of tumor cells. The aim of our study was to investigate the mechanism of LncRNA-HOTAIR in regulating drug resistance of NSCLC cells. Our data indicated that HOTAIR was overexpressed in NSCLC cell lines. Silencing of HOTAIR decreased cell proliferation and increased apoptosis of NSCLC cells (A549). Besides that, HOTAIR shRNA transfection suppressed drug resistance of A549 cells to Crizotinib by more effectively inhibiting cell viability and promoting apoptosis compared with HOTAIR scramble group. Moreover, silencing of HOTAIR decreased the number of LC3+ puncta and the expression of Beclin1, p-ULK1 and the ratio of LC3 II/I/in Crizotinib treated A549 cells, indicating that silencing of HOTAIR decreased drug resistance of NSCLC cells might through inhibiting autophagy via the ULK1 pathway. In order to further prove our conclusion, Rapamycin (Rapa), an autophagy inducer, was used in our study. With the adjunction of Rapa, obvious autophagy was induced by increasing the number of LC3+ puncta, the ratio of LC3 II/I and the expression of p-ULK1 co...Continue Reading

Citations

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