Silencing of Long Non-coding RNA MIAT Sensitizes Lung Cancer Cells to Gefitinib by Epigenetically Regulating miR-34a

Frontiers in Pharmacology
Yunfeng FuRong Gui

Abstract

Long non-coding RNA (lncRNA) myocardial infarction associated transcript (MIAT) was recently identified as oncogene in several cancers. However, the role of MIAT on acquired resistance in lung cancer and the underlying mechanisms remain unclear. Here, we showed that the expression of MIAT in lung cancer tissues was upregulated compared with adjacent tissues. LncRNA MIAT expression was associated with tumor size, lymph node metastasis, distant metastasis and TNM stage. Univariate analysis and multivariate analysis revealed that the lncRNA MIAT to be an independent factor for predicating the prognosis of lung cancer patients. Low lncRNA MIAT have longer overall survival time and progression-free survival time than patients with high lncRNA MIAT expression. Moreover, the knockdown of MIAT significantly sensitized PC9 and gefitinib-resistant PC9 cells to gefitinibin vitroandin vivo, and increased the expression of miR-34a and inactivated PI3K/Akt signaling. MIAT interacted with miR-34a and epigenetically controlled the miR-34a expression by hyper-methylating its promotor. Taken together, our findings demonstrated that knockdown of MIAT by siRNA enhances lung cancer cells to gefitinib through the PI3K/Akt signaling pathway by epigen...Continue Reading

References

Nov 4, 2011·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Norimitsu TanakaShinichiro Miyoshi
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Mar 26, 2015·Translational Lung Cancer Research·Erin L StewartMing-Sound Tsao
Nov 19, 2015·Journal of Clinical Medicine·Yoshimasa SaitoHidetsugu Saito
Dec 15, 2015·Expert Opinion on Therapeutic Targets·Brian D AdamsFrank J Slack
Dec 29, 2015·Gene·Jiangquan LiaoJie Wang
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
May 18, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ying ChenLongbang Chen
Jan 4, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Pei MaLiang Han
Apr 7, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Haihua TianZhaohui Gong
Jun 20, 2017·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Jane ZhaoAndreas G Bader
Jul 3, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hongwei LiBo Yang
Sep 6, 2017·BMC Cancer·Xiaoli PengMantian Mi
Oct 17, 2017·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Man ZhuJiancheng Tu
Oct 27, 2017·Nature Reviews. Cancer·Julia Rotow, Trever G Bivona
Oct 31, 2017·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wei LiKe Shi
Nov 4, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xinli HuangSen Lu

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Citations

Jun 6, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Waisheng ZhongJie Chen
Jan 8, 2020·Current Drug Metabolism·Priya MondalSyed Musthapa Meeran
Mar 19, 2020·Cancer Biotherapy & Radiopharmaceuticals·Suiyang ZhouXianchao Kong
Oct 1, 2019·Surgical Laparoscopy, Endoscopy & Percutaneous Techniques·Chaofeng LiWenyue Wang
Aug 28, 2019·Oncology Letters·Hui-Li WangJia-Hui Yang
Nov 22, 2019·Pathology Oncology Research : POR·Lei DingYanli Li
Feb 18, 2020·Journal of Cellular Physiology·Sanaz NaghizadehBehzad Mansoori
Apr 24, 2020·Cancer Immunology Research·Shouping XuDa Pang
Jan 9, 2021·Briefings in Bioinformatics·Qing-Wen WuChun-Hou Zheng
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Mar 26, 2021·Frontiers in Cell and Developmental Biology·Wen Jess LiDean G Tang
May 8, 2021·Cancer Metastasis Reviews·Sijing LiLi Sun
May 2, 2021·Clinical and Translational Medicine·Yu ChenYouping Deng
Jun 15, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chao-Ming DaHai-Hong Zhang

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Methods Mentioned

BETA
PCR
Assay
Immunoprecipitation
RIP
electrophoresis
Xenograft
xenografts
transfection
Flow cytometry
RNA-seq

Software Mentioned

catRAPID
MIAT
SPSS
GraphPad Prism
MiRcode
FlowJo
RNAplex
Image

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