Silencing of Long Noncoding RNA SNHG6 Inhibits Esophageal Squamous Cell Carcinoma Progression via miR-186-5p/HIF1α Axis

Digestive Diseases and Sciences
Fang DuChenghua Cao


Long noncoding RNA (lncRNA) small nucleolar RNA host gene 6 (SNHG6) has been shown to be upregulated in esophageal squamous cell carcinoma (ESCC). However, its detailed function in ESCC remains unknown. We investigated its specific roles in ESCC cell proliferation, invasion, and migration. Gene expression was evaluated by quantitative reverse transcriptase polymerase chain reaction and western blot. The subcellular localization of lncRNA SNHG6 was determined using subcellular location assay. Luciferase reporter assay and RNA pull-down assay were applied to determine the interaction between lncRNA SNHG6, miR-186-5p, and hypoxia-inducible factor 1α (HIF1α). The cell proliferation, migration, and invasion abilities were evaluated by using cell count kit-8, colony formation assay, and transwell migration and invasion assays. LncRNA SNHG6 and HIF1α were upregulated, while miR-186-5p was downregulated in ESCC tissues and cell lines. Knockdown of lncRNA SNHG6 inhibits ESCC cell proliferation, migration, and invasion. A negative correlation between lncRNA SNHG6 and miR-186-5p expression was found in ESCC tissues. Similarly, there is a positive correlation between lncRNA SNHG6 and HIF1α expression in ESCC tissues. Conversely, miR-186-5p...Continue Reading


Feb 18, 2012·Nature·Mitchell Guttman, John L Rinn
Mar 19, 2013·Cell·Pedro J Batista, Howard Y Chang
May 24, 2013·Chemical Reviews·Haifeng DongXueji Zhang
Apr 2, 2014·Nature Genetics·De-Chen LinH Phillip Koeffler
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Apr 14, 2016·Cancer Cell·Adam M Schmitt, Howard Y Chang
Mar 27, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Gang CaiQing Lan
Apr 3, 2018·Archives of Biochemistry and Biophysics·Jianwei RuanMaoquan Li
Aug 16, 2018·Nature Reviews. Molecular Cell Biology·Luca F R Gebert, Ian J MacRae
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rui LiangHong Ren
Dec 7, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Pengwei LvYunqing Yang
Jan 11, 2019·Journal of Hematology & Oncology·Mu XuShukui Wang
Mar 3, 2019·Journal of Experimental & Clinical Cancer Research : CR·Shanshan DongYong Zeng
Mar 22, 2019·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zhiqiang ZhangMingyu Fan
May 8, 2019·Applied Microbiology and Biotechnology·Chit TamTzi Bun Ng

Related Concepts

Western Blotting
Cell Count
Colony-Forming Units Assay
Gene Expression
Up-Regulation (Physiology)
Disease Progression
Squamous Cell Carcinoma of Esophagus
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