Silencing of Long Noncoding RNA SOX21-AS1 Relieves Neuronal Oxidative Stress Injury in Mice with Alzheimer's Disease by Upregulating FZD3/5 via the Wnt Signaling Pathway

Molecular Neurobiology
Lu ZhangHong-Liang Xu

Abstract

Alzheimer's disease (AD) represents a progressive neurodegenerative disorder characterized by distinctive neuropathological changes. Recently, long noncoding RNAs (lncRNAs) have become a key area of interest due to their potential in AD therapy. Hence, the aim of the current study was to investigate the effect of lncRNA SOX21-AS1 on neuronal oxidative stress injury in mice with AD via the Wnt signaling pathway by targeting FZD3/5. Microarray analysis was performed to screen AD-related differentially expressed genes (DEGs). Following verification of the target relationship between SOX21-AS1 and FZD3/5, the contents of OH-, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were determined, with the expressions of SOX21-AS1, FZD3/5, β-catenin, cyclin D1, and 4-HNE in hippocampal neuron cells subsequently detected. Cell cycle distribution and apoptosis were evaluated. Bioinformatics analysis revealed that SOX21-AS1 was upregulated in AD, while highlighting the co-expression of SOX21-AS1 and FZD3/5 genes and their involvement in the Wnt signaling pathway. AD mice exhibited diminished memory and learning ability, increased rates of MDA, OH-, SOX21-AS1, 4-HNE, and elevated levels of...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Oct 25, 2006·BMC Bioinformatics·André FujitaMari Cleide Sogayar
May 17, 2007·IUBMB Life·Nibaldo C InestrosaMarcela Colombres
Jul 26, 2008·Molecular Neurodegeneration·Nibaldo C Inestrosa, Enrique M Toledo
Aug 16, 2008·Behavioral and Brain Functions : BBF·Makiko KishimotoShigetoshi Kuroda
Mar 20, 2009·Annals of Neurology·Leslie M ShawUNKNOWN Alzheimer's Disease Neuroimaging Initiative
Jul 1, 2007·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Ron BrookmeyerH Michael Arrighi
Jul 17, 2010·Journal of Alzheimer's Disease : JAD·Manjeet SinghCharles Ramassamy
Sep 9, 2010·International Journal of Cancer. Journal International Du Cancer·Maria FerlettaBengt Westermark
Oct 23, 2010·Molecular Psychiatry·K N ManolopoulosA Barthel
Sep 8, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Satoru MatsudaHideyuki Okano
Nov 29, 2012·BMB Reports·Reo Maruyama, Hiromu Suzuki
Mar 13, 2013·The Journal of Clinical Investigation·Valentina SasselliVassilis Pachnis
Aug 1, 2013·Molecular Medicine Reports·Xiang-Xiang Zhou, Xin Wang
Aug 29, 2013·Oxidative Medicine and Cellular Longevity·Yan Zhao, Baolu Zhao
Dec 7, 2013·Cancers·Murugan SubramanianAshish Lal
Apr 14, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Hui QiangChen Zhang
May 1, 2015·Expert Opinion on Drug Discovery·Daniela PuzzoOttavio Arancio
Aug 14, 2015·Current Topics in Medicinal Chemistry·Zijian Zhang
Sep 9, 2015·Genetics and Molecular Research : GMR·Y Y ZhangL M Guo
Dec 8, 2015·Biochemistry. Biokhimii︠a︡·N K IsaevV P Skulachev
May 3, 2016·Journal of Korean Medical Science·Jianping XiangJinyu Xiang

❮ Previous
Next ❯

Citations

Sep 24, 2020·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Elizabeth E BlueAlexander P Reiner
Mar 19, 2020·Metabolic Brain Disease·Shamseddin AhmadiSteven Bradburn
May 18, 2020·Cell Death & Disease·Xing DuQifa Li
Apr 9, 2020·Journal of Biomedical Science·Yi-Ying Wu, Hung-Chih Kuo
Jun 2, 2020·Frontiers in Aging Neuroscience·Yunying Yang, Zhentao Zhang
Jul 18, 2020·Molecular Neurodegeneration·Tiantian GuoYingjun Zhao
Nov 13, 2020·Frontiers in Neurology·Xuewen XiaoBin Jiao
Nov 12, 2020·Antioxidants·Ana Gámez-ValeroEulàlia Martí
Jun 21, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
May 1, 2021·International Journal of Molecular Sciences·Chisato Kinoshita, Koji Aoyama
Mar 17, 2020·Computers in Biology and Medicine·Turki Turki, Y-H Taguchi
Aug 21, 2021·Journal of Molecular Neuroscience : MN·Soudeh Ghafouri-FardMohammad Samadian
Nov 9, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sajad NajafiShahin Aghamiri

❮ Previous
Next ❯

Methods Mentioned

BETA
chip
transfection
electrophoresis
flow cytometry
MDA

Software Mentioned

Multi Experiment Matrix ( MEM
Image Pro Plus
SPSS
R
Affy installation
Limma

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Alzheimer's Disease: RNA Regulation

RNA regulation involves several mechanisms that are used by cells to decrease or increase the production of RNA. Disruption of RNA regulatory processes has been associated with Alzheimer's disease (AD). Here are the latest discoveries pertaining to RNA regulation and AD.

Alzheimer's Disease: Animal Models

Alzheimer's disease is a neurodegenerative disease which can be studied using various experimental systems. This feed focuses on animal models used for Alzheimer's disease research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.