Silencing of Mustn1 inhibits myogenic fusion and differentiation.

American Journal of Physiology. Cell Physiology
Cheng LiuMichael Hadjiargyrou

Abstract

Mustn1 (Mustang, musculoskeletal temporally activated novel gene) was originally identified in fracture callus tissue, but its greatest expression is detected in skeletal muscle. Thus, we conducted experiments to investigate the expression and function of Mustn1 during myogenesis. Temporally, quantitative real-time PCR analysis of muscle samples from embryonic day 17 to 12 mo of age reveals that Mustn1 mRNA expression is greatest at 3 mo of age and beyond, consistent with the expression pattern of Myod. In situ hybridization shows abundant Mustn1 expression in somites and developing skeletal muscles, while in adult muscle, Mustn1 is localized to some peripherally located nuclei. Using RNA interference (RNAi), we investigated the function of Mustn1 in C2C12 myoblasts. Though silencing Mustn1 mRNA had no effect on myoblast proliferation, it did significantly impair myoblast differentiation, preventing myofusion. Specifically, when placed in low-serum medium for up to 6 days, Mustn1-silenced myoblasts elongated poorly and were mononucleated. In contrast, control RNAi-treated and parental myoblasts presented as large, multinucleated myotubes. Further supporting the morphological observations, immunocytochemistry of Mustn1-silenced ...Continue Reading

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Citations

Nov 10, 2012·Cell and Tissue Research·Yan WangStephen E Alway
Jul 10, 2012·Journal of Physiology and Biochemistry·You Bing YangInho Hwang
Nov 5, 2013·BMC Genomics·Nicholas J PooleySamuel A M Martin
Sep 12, 2014·Molecular Biology of the Cell·Aymeric Ravel-ChapuisBernard J Jasmin
Apr 16, 2011·Journal of Vascular Surgery·Richard D KenagyAlexander W Clowes
Mar 27, 2013·International Journal of Molecular Sciences·Juan LiQing Zhu
Jan 13, 2018·International Journal of Molecular Sciences·Michael Hadjiargyrou
Sep 12, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Paula Suarez-BreguaJosep Rotllant
Oct 10, 2020·Animal Biotechnology·Zhixiu WangGuobin Chang

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