Silencing of RpATG6 impaired the yolk accumulation and the biogenesis of the yolk organelles in the insect vector R. prolixus

PLoS Neglected Tropical Diseases
Priscila H VieiraIsabela B Ramos

Abstract

In oviparous animals, the egg yolk is synthesized by the mother in a major metabolic challenge, where the different yolk components are secreted to the hemolymph and delivered to the oocytes mostly by endocytosis. The yolk macromolecules are then stored in a wide range of endocytic-originated vesicles which are collectively referred to as yolk organelles and occupy most of the mature oocytes cytoplasm. After fertilization, the contents of these organelles are degraded in a regulated manner to supply the embryo cells with fundamental molecules for de novo synthesis. Yolk accumulation and its regulated degradation are therefore crucial for successful development, however, most of the molecular mechanisms involved in the biogenesis, sorting and degradation of targeted yolk organelles are still poorly understood. ATG6 is part of two PI3P-kinase complexes that can regulate the recruitment of the endocytic or the autophagy machineries. Here, we investigate the role of RpATG6 in the endocytosis of the yolk macromolecules and in the biogenesis of the yolk organelles in the insect vector Rhodnius prolixus. We found that vitellogenic females express high levels of RpATG6 in the ovaries, when compared to the levels detected in the midgut ...Continue Reading

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Citations

Aug 28, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Larissa Bomfim, Isabela Ramos
Mar 12, 2021·Development Genes and Evolution·Agustina PascualRolando Rivera Pomar
Nov 8, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Gleidson Silva-OliveiraKatia C Gondim

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Datasets Mentioned

BETA
KQ0344093

Methods Mentioned

BETA
PCR
transmission electron microscopy
electron microscopy

Software Mentioned

Adobe Photoshop
SIA
Bio Edit software
Vector Base
BLAST
PFAM
GraphPad
Prism
SIAS
SoftMax Pro

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