Silencing of the UCHL1 gene in giant cell tumors of bone

International Journal of Cancer. Journal International Du Cancer
Jörg FellenbergDaniela Witte

Abstract

Giant cell tumors are heterogeneous tumors consisting of multinucleated giant cells, fibroblast-like stromal cells and mononuclear histiocytes. The stromal cells have been identified as the neoplastic cell population, which promotes the recruitment of histiocytes and the formation of giant cells. Strong evidence exists that these cells develop from mesenchymal stem cells (MSCs) but little is known about the molecular mechanisms involved in GCT tumorigenesis. The aim of our study was the identification of cancer-related genes differentially expressed in GCTs compared to MSCs in order to identify possible targets for aberrant promoter methylation, which may contribute to MSC transformation and GCT development. Gene expression of 440 cancer-related genes was analyzed by DNA microarrays in GCT stromal cells and bone marrow-derived MSCs (BMSCs) isolated from the same patient (n = 3) to avoid interindividual variations. Differential expression was identified for 14 genes, which could be confirmed by quantitative PCR in further 21 GCT and 10 BMSC samples. The most pronounced difference in gene expression was detected for UCHL1, an important regulator of the ubiquitin proteasome pathway. Methylation-specific PCR and bisulfite sequencin...Continue Reading

References

Jul 1, 1994·Journal of Cellular Biochemistry·D Robinson, T A Einhorn
Oct 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I E JamesM Gowen
May 9, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·G GamberiP Picci
Mar 24, 2000·Trends in Genetics : TIG·S B Baylin, J G Herman
Apr 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G J AtkinsD M Findlay
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Aug 15, 2001·Journal of Cancer Research and Clinical Oncology·M WüllingE Kaiser
Jul 17, 2003·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Dror RobinsonZvi Nevo
Aug 12, 2003·International Journal of Radiation Oncology, Biology, Physics·Jimmy J CaudellAlan W Yasko
Aug 13, 2003·Human Molecular Genetics·Hitoshi OsakaKeiji Wada
Sep 10, 2003·The Annals of Thoracic Surgery·Kuang-Tai KuoLiang-Shun Wang
Mar 5, 2004·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G GamberiP Picci
Sep 28, 2004·Virchows Archiv : an International Journal of Pathology·M WuellingE Kaiser
Jul 22, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Aparna Mani, Edward P Gelmann
Nov 25, 2005·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Atsushi MurataKatsuro Tomita
Apr 28, 2006·International Journal of Cancer. Journal International Du Cancer·Eriko Okochi-TakadaToshikazu Ushijima
Oct 3, 2006·International Orthopaedics·Mathias Werner
Feb 27, 2007·Cell·Peter A Jones, Stephen B Baylin
Sep 26, 2007·Human Molecular Genetics·Manel Esteller
May 31, 2008·International Journal of Cancer. Journal International Du Cancer·Yutaka TokumaruDavid Sidransky
Jul 31, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jun YuJoseph J Y Sung
Aug 4, 2009·Journal of Cellular Biochemistry·Katie L PricolaRocky S Tuan

❮ Previous
Next ❯

Citations

Aug 6, 2011·American Journal of Respiratory and Critical Care Medicine·Sumegha MitraJeffrey R Jacobson
Dec 3, 2014·Pharmacology & Therapeutics·Padraig D'ArcyStig Linder
Oct 16, 2012·Bone·Robert W Cowan, Gurmit Singh
Sep 23, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Haiyan QinXiaofeng Huang
Nov 20, 2016·Journal of Cellular Biochemistry·Carol P Y LauShekhar Madhukar Kumta
Mar 25, 2020·Cell Biochemistry and Function·Ewa MatuszczakAdam Hermanowicz
Jun 6, 2020·Cancer Letters·Federica Scotto di CarloFernando Gianfrancesco

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.

Cancer Epigenetics & Methyl-CpG (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. Here is the latest research on cancer epigenetics and methyl-CpG binding proteins including ZBTB38.

Cancer Epigenetics

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.